利用基于臭氧的气相化学发光（CL）直接测量酶产生的一氧化氮（NO）的速率。
Direct measurement of nitric oxide (NO) production rates from enzymes using ozone-based gas-phase chemiluminescence (CL).
机构信息
Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15261, USA; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261, USA.
Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15261, USA; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, 15261, USA.
出版信息
Nitric Oxide. 2021 Dec 1;117:60-71. doi: 10.1016/j.niox.2021.10.001. Epub 2021 Oct 13.
Nitric oxide (NO) chemiluminescence detectors (CLDs) are specialized and sensitive spectroscopic instruments capable of directly measuring NO flux rates. NO CLDs have been instrumental in the characterization of mammalian nitrite-dependent NO synthases. However, no detailed description of NO flux analysis using NO CLD is available. Herein, a detailed review of the NO CL methodology is provided with guidelines for measuring NO-production rates from aqueous samples, such as isolated enzymes or protein homogenates. Detailed description of the types of signals one can encounter, data processing, and potential pitfalls related to NO flux measurements will also be covered.
一氧化氮（NO）化学发光探测器（CLD）是一种专门的、敏感的光谱仪器，能够直接测量 NO 通量率。NO CLD 在哺乳动物亚硝酸盐依赖型一氧化氮合酶的表征中发挥了重要作用。然而，目前还没有关于使用 NO CLD 进行 NO 通量分析的详细描述。本文提供了一份关于 NO CL 方法学的详细综述，并提供了从水样（如分离的酶或蛋白质匀浆）中测量 NO 产生率的指南。还将详细描述可能遇到的信号类型、数据处理以及与 NO 通量测量相关的潜在陷阱。
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