School of Civil Engineering, Chang'an University, The Middle Section of the South 2(nd) Ring Road, 710064, Xi'an, Shaanxi Province, China; Key Laboratory of Water Supply & Sewage Engineering, Ministry of Housing and Urban-rural Development, Chang'an University, The Middle Section of the South 2(nd) Ring Road, 710064, Xi'an, Shaanxi Province, China.
Key Laboratory of Water Supply & Sewage Engineering, Ministry of Housing and Urban-rural Development, Chang'an University, The Middle Section of the South 2(nd) Ring Road, 710064, Xi'an, Shaanxi Province, China; School of Water and Environment, Chang'an University, The Middle Section of the South 2(nd) Ring Road, 710064, Xi'an, Shaanxi Province, China; Key Laboratory of Environmental Protection & Pollution and Remediation of Water and Soil of Shaanxi Province, Chang'an University, The Middle Section of the South 2(nd) Ring Road, 710064, Xi'an, Shaanxi Province, China.
Bioresour Technol. 2022 Jan;343:126116. doi: 10.1016/j.biortech.2021.126116. Epub 2021 Oct 12.
A heterotrophic nitrification- aerobic denitrification (HNAD) bacterium, Acinetobacter junii ZHG-1, was isolated, meanwhile, the optimal conditions for the strain were evaluated, moreover, the influence mechanism of the C/N ratio on the HNAD process was investigated from the perspective of electron transport and energy level. The increasing of C/N ratio enhanced the reduced/oxidized nicotinamide adenine dinucleotide (NADH/NAD) ratio, NADH concentration, electron transport system activity (ETSA), ATP content, as well as enzymes activities, consequently, the HNAD performance of the strain can be improved, however, when the C/N ratio was higher than 30, the activities of enzymes relating to the HNAD process and the ETSA had reached the maximum, which might limit the further improvement of the nitrogen removal with the increasing of C/N ratio. As the interaction between different biochemical reactions in HNAD process, more efforts should be devoted to the influent mechanism of different environmental factors on the HNAD process.
一株好氧反硝化异养硝化菌——琼氏不动杆菌 ZHG-1 被分离出来，同时评估了该菌株的最佳条件，此外，还从电子传递和能量水平的角度研究了 C/N 比对 HNAD 过程的影响机制。C/N 比的增加提高了还原型/氧化型烟酰胺腺嘌呤二核苷酸（NADH/NAD）比、NADH 浓度、电子传递系统活性（ETSA）、ATP 含量以及酶活性，从而提高了菌株的 HNAD 性能，然而，当 C/N 比高于 30 时，与 HNAD 过程和 ETSA 相关的酶的活性已经达到最大值，这可能限制了随着 C/N 比的增加进一步提高氮的去除率。由于 HNAD 过程中不同生化反应之间的相互作用，需要更多的努力来研究不同环境因素对 HNAD 过程的影响机制。
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