Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Bioresour Technol. 2022 Jan;344(Pt A):126184. doi: 10.1016/j.biortech.2021.126184. Epub 2021 Oct 25.
Conventional autotrophic nitrification process is difficult to treat high-temperature wastewater with high-strength ammonia. In this study, a high-temperature (50 °C) biofilm system based on heterotrophic nitrification and aerobic denitrification (HNAD) was established. The results showed that the HNAD process was high temperature resistant, and the nitrogen removal performance, pathway and microbial mechanism varied remarkably at different temperatures. The high-temperature system showed excellent nitrogen and COD removal capacities at 50 °C. Ammonia oxidation was mainly undertaken by heterotrophic nitrification, while anoxic and aerobic pathways worked in concert for denitrification. High-throughput sequencing indicated that heterotrophic nitrifying bacteria (8.58%) and denitrifying bacteria (52.88%) were dominant at 50 °C. Metagenomic analysis further suggested that the carbon metabolism was up-regulated in response to the increasing temperature, so more energy was generated, thereby promoting the HNAD-related nitrogen removal pathways. The study revealed the microbial mechanism of HNAD at high temperature and provided new insights into high-temperature biological nitrogen removal.
传统的自养硝化工艺很难处理高温、高氨强度的废水。本研究建立了一种基于异养硝化和好氧反硝化（HNAD）的高温（50°C）生物膜系统。结果表明，HNAD 工艺具有耐高温性，在不同温度下，脱氮性能、途径和微生物机制差异显著。高温系统在 50°C 时表现出优异的氮和 COD 去除能力。氨氧化主要由异养硝化完成，而缺氧和好氧途径协同进行反硝化。高通量测序表明，异养硝化菌（8.58%）和反硝化菌（52.88%）在 50°C 时占优势。宏基因组分析进一步表明，碳代谢随着温度的升高而上调，从而产生更多的能量，从而促进与 HNAD 相关的氮去除途径。本研究揭示了高温下 HNAD 的微生物机制，为高温生物脱氮提供了新的见解。
