Medical Biochemistry Department, Medical Research Division, National Research Centre, Cairo, Egypt.
Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Cairo, Egypt.
Environ Toxicol. 2022 Feb;37(2):212-223. doi: 10.1002/tox.23390. Epub 2021 Oct 16.
α-Solanine is a natural toxic glycoalkaloid produced in some species of the Solanaceae family with antiproliferative activity in various cancers.
This study aimed to investigate the effect of α-solanine on the oxidative stress status in human hepatocellular carcinoma HepG2 cells and to evaluate its influence on microRNAs (miRNAs) associated with oxidative stress and NF-κB regulation.
The prooxidant effect of α-solanine was tested by the decay rate of the fluorescent probe, β-phycoerythrin, and by measuring malondialdehyde, reduced Glutathione, catalase, and superoxide dismutase following treatment of HepG2 cells with low doses of α-solanine. Immunocytochemical techniques were used to detect mitochondrial membrane potential (ΔΨm) and NF-κB protein. The gene expression of NF-κB and miRNAs was evaluated by real-time PCR.
α-Solanine is a prooxidant that causes a rapid decay in the fluorescence intensity of β-phycoerythrin. It induces oxidative stress-related alterations such as increased lipid peroxidation and reduced antioxidant markers. Oxidative stress induced by α-solanine was mediated by decreased ΔΨm, increased NF-κB expression, upregulation of miRNAs that control oxidative stress by regulating the NF-κB pathway, and downregulation of oncogenic miRNAs that inhibit the NF-κB pathway.
α-Solanine-induced oxidative stress is mediated by alterations in the NF-κB pathway with a detected crosstalk between α-solanine treatment and the expression of oxidative stress-responsive miRNAs.
α-茄碱是茄科某些物种中产生的一种天然有毒糖苷生物碱，对多种癌症具有抗增殖活性。
本研究旨在探讨α-茄碱对人肝癌 HepG2 细胞氧化应激状态的影响，并评价其对与氧化应激和 NF-κB 调节相关的 microRNAs（miRNAs）的影响。
通过荧光探针β-藻红蛋白的衰减率以及测定低剂量α-茄碱处理 HepG2 细胞后丙二醛、还原型谷胱甘肽、过氧化氢酶和超氧化物歧化酶来测试 α-茄碱的促氧化作用。免疫细胞化学技术用于检测线粒体膜电位（ΔΨm）和 NF-κB 蛋白。通过实时 PCR 评估 NF-κB 和 miRNAs 的基因表达。
α-茄碱是一种促氧化剂，可导致β-藻红蛋白的荧光强度迅速衰减。它引起与氧化应激相关的改变，如脂质过氧化增加和抗氧化标志物减少。α-茄碱诱导的氧化应激是通过降低ΔΨm、增加 NF-κB 表达、上调通过调节 NF-κB 途径控制氧化应激的 miRNAs 以及下调抑制 NF-κB 途径的致癌 miRNAs 来介导的。
α-茄碱诱导的氧化应激是通过 NF-κB 途径的改变介导的，α-茄碱处理与氧化应激反应性 miRNAs 的表达之间存在检测到的串扰。
