大肠杆菌K-12 lexA突变体单克隆中细胞分裂的分析。
Analysis of cell division in single clones of the Escherichia coli K-12 lexA mutant.
作者信息
Howe W E, Mount D W
出版信息
J Bacteriol. 1978 Mar;133(3):1278-81. doi: 10.1128/jb.133.3.1278-1281.1978.
Abstract
The growth of clones of lexA mutant and lexA+ cells was analyzed. During normal growth lexA mutant clones frequently divided early, producing smaller newborn cells than the lexA+ clones. Some newborn cells in the lexA clones did not elongate or divide at all, a response that was never observed in the lexA+ clones. When starved for thymidine, most of the lexA mutant clones elongated and subsequently divided. The majority of lexA+ clones also elongated but did not divide. The above results suggest that one of the functions of the lexA+ gene is coordination of DNA repair with cell division.
摘要
对lexA突变体和lexA+细胞的克隆生长进行了分析。在正常生长过程中，lexA突变体克隆经常过早分裂，产生的新生细胞比lexA+克隆的新生细胞小。lexA克隆中的一些新生细胞根本不伸长或分裂，这种反应在lexA+克隆中从未观察到。当缺乏胸苷时，大多数lexA突变体克隆伸长并随后分裂。大多数lexA+克隆也伸长但不分裂。上述结果表明，lexA+基因的功能之一是协调DNA修复与细胞分裂。
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