Division of Materials Analysis Research, Korea Basic Science Institute, 169-148, Gwahak-ro, Yuseong-Gu, Daejeon, 34133, Republic of Korea.
Department of Bionano Engineering, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Republic of Korea.
Mikrochim Acta. 2021 Oct 17;188(11):382. doi: 10.1007/s00604-021-05032-4.
A sensitive on-site mercury sensing platform was developed for simple and effective monitoring of mercury levels in the field. The simple and practical mercury detection system was designed by integrating an Au nanoparticle-PEG hydrogel block nanozyme (Au-HBNz) into a polymer film-based colorimetric device. Upon addition of Hg ions, Au-HBNz exhibited excellent peroxidase-like activity, catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine into a blue-colored product, which has a maximum absorbance at 652 nm. The resulting color intensity change was evaluated using a smartphone for simple and rapid Hg detection with a broad detection range (0.008-20 μg∙mL) and a linear concentration-response relationship (R = 0.96). The detection limit (1.10 ng∙mL) was lower than the maximum permissible Hg levels in drinking water set by the World Health Organization (6 ng∙mL) and U.S. Environmental Protection Agency (2 ng∙mL). The recoveries of Hg determination in river water by spiking Hg samples ranged from 92 to 106%, which indicated high validity and applicability of the Hg detection system for field measurements. Thus, the developed sensor enables highly selective and efficient real-time monitoring of Hg.
开发了一种灵敏的现场汞传感平台，用于简单有效地监测现场的汞含量。通过将金纳米粒子-聚乙二醇水凝胶块纳米酶（Au-HBNz）集成到基于聚合物膜的比色装置中，设计了简单实用的汞检测系统。加入 Hg 离子后，Au-HBNz 表现出优异的过氧化物酶样活性，可催化 3,3',5,5'-四甲基联苯胺氧化成蓝色产物，其在 652nm 处具有最大吸收。使用智能手机评估所得颜色强度变化，可实现简单快速的 Hg 检测，具有较宽的检测范围（0.008-20μg·mL）和线性浓度响应关系（R=0.96）。检测限（1.10ng·mL）低于世界卫生组织（6ng·mL）和美国环境保护署（2ng·mL）规定的饮用水中最大允许 Hg 水平。通过向河水样本中添加 Hg 样品进行 Hg 测定的回收率在 92%至 106%之间，这表明 Hg 检测系统对于现场测量具有较高的有效性和适用性。因此，所开发的传感器可实现对 Hg 的高选择性和高效实时监测。
