Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, Incheon 402-751, South Korea.
Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 402-751, South Korea.
Anal Chem. 2020 Apr 7;92(7):4917-4925. doi: 10.1021/acs.analchem.9b05025. Epub 2020 Mar 20.
It is highly challenging to develop fast and sensitive fluorescent methods for monitoring organic mercury in purely aqueous solutions as well as live cells. Especially, selective fluorescent detection of methylmercury over inorganic mercury ions has not been reported. We developed a fast and sensitive fluorescent detection method for Hg ions as well as methylmercury using an amino acid-based fluorescent probe () and SDS micelles. The fluorescent probe in SDS micelles detected sensitively and selectively Hg ions and methylmercury among 16 metal ions in purely aqueous solution by the enhancement of the red emission at 575 nm, and the detection of methylmercury was completed within 1 min. The probe in SDS micelles with EDTA showed highly sensitive and selective turn on detection for methylmercury over Hg. The limit of detection was 9.1 nM for Hg (1.8 ppb, = 0.989) and 206 nM for CHHg ( = 0.997). rapidly penetrated live cells and detected intracellular Hg ions as well as CHHg by the enhancement of both red emissions and green emissions. Subsequent treatment of EDTA into the cell confirmed the selective detection of methylmercury in the cells. The present work indicated that the fluorescent probe with micelle systems provided a fast, sensitive, and selective detection method for monitoring inorganic mercury as well as methyl mercury.
开发用于在纯水溶液和活细胞中监测有机汞的快速灵敏荧光方法极具挑战性。特别是，尚未报道过对甲基汞离子选择性荧光检测的方法。我们开发了一种使用基于氨基酸的荧光探针 () 和 SDS 胶束的快速灵敏的荧光检测方法，用于检测 Hg 离子和甲基汞。在 SDS 胶束中，荧光探针在纯水溶液中对 16 种金属离子中的 Hg 离子和甲基汞具有灵敏和选择性的检测，通过增强 575nm 处的红色发射，并且可以在 1 分钟内完成对甲基汞的检测。SDS 胶束中的探针与 EDTA 结合后，对 Hg 显示出高度灵敏和选择性的开启检测，对甲基汞的检测限为 9.1 nM（1.8 ppb， = 0.989）和 206 nM（ = 0.997）。该探针快速穿透活细胞，通过增强红色和绿色发射来检测细胞内 Hg 离子和 CHHg。随后向细胞中加入 EDTA 处理，证实了细胞中甲基汞的选择性检测。本工作表明，带有胶束体系的荧光探针为监测无机汞和甲基汞提供了一种快速、灵敏、选择性的检测方法。
