Epigenetics and Chromatin Biology Group, Gupta Lab, Cancer Research Institute, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, MH, 410210, India.
Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, MH, India.
Mol Biol Rep. 2021 Dec;48(12):7967-7974. doi: 10.1007/s11033-021-06828-6. Epub 2021 Oct 16.
Reference genes are considered stable genes and are used for normalizing the gene expression profile across different cell types; as well as, in normal and diseased samples. However, these gene associates with different biological processes, and hence expression vary in different pathological conditions. Therefore, in the present study, eight different reference genes were used and compared to identify common reference gene usable for an array of different cell types and human cancers.
The expression stability of the eight reference genes across eleven normal and cancerous tissues was confirmed through real time-qPCR. Ribosomal protein S13 (RPS13) was found to be a common and stable reference gene across intra- and inter-comparison between various normal and tumor tissue types. Further, TCGA data analysis across and between normal and tumor tissue types also showed minimum deviation in expression of RPS13 gene out of eight routinely used reference genes.
RPS13 is the common stable reference gene in normalization for gene expression based analysis in cancer research.
参考基因被认为是稳定的基因，用于标准化不同细胞类型以及正常和患病样本中的基因表达谱。然而，这些基因与不同的生物过程相关联，因此在不同的病理条件下表达会有所不同。因此，在本研究中，使用了 8 种不同的参考基因，并进行了比较，以确定可用于一系列不同细胞类型和人类癌症的常见参考基因。
通过实时 qPCR 证实了 8 个参考基因在 11 种正常和癌组织中的表达稳定性。核糖体蛋白 S13（RPS13）被发现是一种常见且稳定的参考基因，可用于各种正常和肿瘤组织类型的内比较和间比较。此外，TCGA 数据分析还显示，在 8 种常用参考基因中，RPS13 基因的表达变化最小。
RPS13 是癌症研究中基于基因表达分析的常用稳定参考基因。
