Department of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, Beijing, 100191, China.
Institute of Sports Medicine, Peking University, Beijing, 100191, China.
Adv Mater. 2021 Dec;33(52):e2104758. doi: 10.1002/adma.202104758. Epub 2021 Oct 24.
Osteoarthritis (OA) is a musculoskeletal disorder disease affecting about 500 million people worldwide and mesenchymal sem cells (MSCs) therapy has been demonstrated as a potential strategy to treat OA. However, the shear forces during direct injection and the harsher shear condition of OA environments would lead to significant cell damage and inhibit the therapeutic efficacy. Herein, DNA supramolecular hydrogel has been applied as delivering material for MSCs to treat severe OA model, which perform extraordinary protection in MSCs against the shear force both in vitro and in vivo. It is demonstrated that the DNA supramolecular hydrogel can promote formation of quality cartilage, reduce osteophyte, and normalize subchondral bone under the high friction condition of OA, whose molecular mechanisms underlying therapeutic effects are also investigated. It can be anticipated that DNA supramolecular hydrogel would be a promising cell delivery system for multiple potential MSCs therapy.
骨关节炎（OA）是一种影响全球约 5 亿人的肌肉骨骼疾病，间充质干细胞（MSCs）疗法已被证明是治疗 OA 的一种有潜力的策略。然而，直接注射过程中的切变力和 OA 环境更苛刻的剪切条件会导致细胞的显著损伤，从而抑制治疗效果。在此，DNA 超分子水凝胶被用作 MSC 的输送材料，以治疗严重的 OA 模型，在体外和体内对 MSC 抵抗切变力都具有非凡的保护作用。研究表明，DNA 超分子水凝胶可以促进软骨的形成，减少骨赘，以及在 OA 的高摩擦条件下使软骨下骨恢复正常，其治疗效果的分子机制也得到了研究。可以预期，DNA 超分子水凝胶将成为一种有前途的细胞输送系统，用于多种潜在的 MSC 治疗。
