Department of Biochemistry & Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, 150086, China.
Department of Emergency Surgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Epigenomics. 2021 Oct;13(20):1591-1605. doi: 10.2217/epi-2021-0293. Epub 2021 Oct 18.
To explore the role and mechanism of long noncoding RNA AC245100.4 and in prostate cancer (PCa). RNA-sequencing analysis was used to detect the downstream genes of AC245100.4. A series of gain- and loss-of-function approaches were used to investigate the roles of AC245100.4 and . RNA immunoprecipitation was performed to examine the interaction between AC245100.4 and STAT3. AC245100.4 was significantly upregulated in PCa cells and tissues. Knockdown of AC21500.4 significantly inhibited the tumorigenesis of PCa cells. Mechanistically, AC245100.4 deregulated the transcription of via increasing p-STAT3, which acted as a transcriptional repressor of . Knockdown of long noncoding RNA AC245100.4 inhibits the tumorigenesis of PCa cells via the STAT3/ axis.
探讨长链非编码 RNA AC245100.4 和 在前列腺癌（PCa）中的作用和机制。使用 RNA 测序分析来检测 AC245100.4 的下游基因。使用一系列增益和功能丧失方法来研究 AC245100.4 和 的作用。进行 RNA 免疫沉淀实验以检查 AC245100.4 和 STAT3 之间的相互作用。AC245100.4 在 PCa 细胞和组织中明显上调。敲低 AC21500.4 显著抑制了 PCa 细胞的肿瘤发生。在机制上，AC245100.4 通过增加 p-STAT3 来调节 的转录，p-STAT3 作为 的转录抑制因子。敲低长链非编码 RNA AC245100.4 通过 STAT3/ 轴抑制 PCa 细胞的肿瘤发生。
