Department of Burns & Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China.
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410008, PR China.
Epigenomics. 2021 Nov;13(22):1817-1829. doi: 10.2217/epi-2021-0284. Epub 2021 Oct 18.
To reveal the alterations of tRNA-derived small RNA (tsRNA) expression profiles induced by hyperbaric oxygen (HBO) treatment in diabetic foot ulcers (DFUs) and investigate new therapeutic targets. tsRNA sequencing was employed in normal skin tissue, in DFUs, and after HBO treatment groups. A quantitative real-time PCR was used to validate tsRNA sequencing results and their targets levels. Bioinformatics analysis was performed to reveal their therapeutic functions in DFUs. A total of 22 tsRNAs were differentially expressed in the three groups. Three selected tsRNAs were validated by quantitative real-time PCR for further analysis, which were all significantly overexpressed in DFU while being normally expressed after HBO treatment. Bioinformatics analysis disclosed that these tsRNAs may play therapeutic roles through the regulation of the Wnt signaling pathway. tsRNAs may be novel useful targets for HBO to treat DFUs.
为了揭示高压氧（HBO）治疗糖尿病足溃疡（DFU）时诱导的 tRNA 衍生的小 RNA（tsRNA）表达谱的改变，并研究新的治疗靶点。对正常皮肤组织、DFU 组织和 HBO 治疗组进行了 tsRNA 测序。采用实时定量 PCR 验证 tsRNA 测序结果及其靶标水平。进行生物信息学分析以揭示它们在 DFU 中的治疗功能。三组之间共有 22 个 tsRNAs 表达差异。通过定量实时 PCR 验证了三个选定的 tsRNA 进行进一步分析，发现它们在 DFU 中均显著过表达，而在 HBO 治疗后则正常表达。生物信息学分析表明，这些 tsRNAs 可能通过调节 Wnt 信号通路发挥治疗作用。tsRNA 可能是 HBO 治疗 DFU 的新的有用靶点。
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