Boutin J A
J Biochem Biophys Methods. 1986 Oct;13(3):171-8. doi: 10.1016/0165-022x(86)90090-4.
The non-ionic detergent Triton X-100 is shown to inhibit the spontaneous oxidation of NAD(P)H associated with rat liver microsomes. Advantage of this observation is taken to measure different microsomal NAD(P)H-dependent oxidoreductase activities such as 3-alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase and various xenobiotic oxidoreductases. This inhibition provides an easy method for the screening of the under-investigated microsomal oxidoreductive metabolism of xenobiotics.
非离子去污剂曲拉通X-100被证明可抑制与大鼠肝微粒体相关的NAD(P)H的自发氧化。利用这一观察结果来测量不同的微粒体NAD(P)H依赖性氧化还原酶活性，如3-α-羟基类固醇脱氢酶、二氢二醇脱氢酶和各种外源性生物氧化还原酶。这种抑制作用为筛选研究不足的外源性生物微粒体氧化还原代谢提供了一种简便方法。
