Han Guangtao, Zhang Yubiao, Liu Ting, Li Jianping, Li Haohuan
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Department of Orthopedics, Hospital of Shenmu, Shenmu, Shaanxi, 719300, P.R. China.
J Cancer. 2021 Aug 30;12(21):6383-6392. doi: 10.7150/jca.54602. eCollection 2021.
Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active components in panax notoginseng and were reported to be capable of inhibiting the growth of several tumors both in vitro and in vivo. However, its effects on osteosarcoma have not been studied yet, which is addressed in this study for the first time. Our results indicated that PNS can inhibit proliferation, invasion and migration of the osteosarcoma cells, while promoting their apoptosis simultaneously. Specifically, PNS caused an increase in mitochondrial membrane potential and the amount of reactive oxygen species. The cell cycle in osteosarcoma cells was arrested in the G0 / G1 phase after PNS treatment. The expression of p53 and other apoptosis-related mitochondrial proteins were promoted. Nevertheless, it was observed that autophagy became less active in osteosarcoma cells when PNS was administered. In a word, PNS were of potential therapeutic significance for osteosarcoma.
骨肉瘤是最常见的原发性骨恶性肿瘤，目前针对骨肉瘤的化疗疗程常常会给患者带来严重并发症。因此，有必要研发副作用更少的新型有效抗肿瘤药物。三七皂苷（PNS）是三七中的活性成分，据报道其在体外和体内均能抑制多种肿瘤的生长。然而，其对骨肉瘤的影响尚未见研究报道，本研究首次对此进行了探讨。我们的结果表明，PNS能抑制骨肉瘤细胞的增殖、侵袭和迁移，同时促进其凋亡。具体而言，PNS导致线粒体膜电位和活性氧含量增加。PNS处理后，骨肉瘤细胞的细胞周期停滞在G0 / G1期。p53及其他与凋亡相关的线粒体蛋白的表达增加。然而，观察到给予PNS时骨肉瘤细胞中的自噬活性降低。总之，PNS对骨肉瘤具有潜在的治疗意义。
