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环状RNA hsa_circ_0001306作为一种竞争性内源性RNA，通过吸附miR-527来调控肝细胞癌中FBXW7的表达。

Circular RNA hsa_circ_0001306 Functions as a Competing Endogenous RNA to Regulate FBXW7 Expression by Sponging miR-527 in Hepatocellular Carcinoma.

作者信息

Wu Yufan, Fan Taihe, Zhao Yubin, Hu Rongkuan, Yan Dongdong, Sun Ding, Gao Ling, Qin Lei, Xue Xiaofeng

机构信息

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215000, JiangSu Province, China.

Kunshan Hospital of Traditional Chinese Medicine, Kunshan, JiangSu Province, China.

出版信息

J Cancer. 2021 Sep 9;12(21):6531-6542. doi: 10.7150/jca.61381. eCollection 2021.

DOI:10.7150/jca.61381
PMID:34659544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8489137/
Abstract

Hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide. Circular RNAs (circRNAs) have been reported to regulate many types of cancers, including HCC. The purpose of this study was to investigate the potential roles of hsa_circ_0001306 in HCC. Firstly, the downregulation of hsa_circ_0001306 was identified by high‑throughput RNA sequencing and further verified by qRT-PCR. Secondly, we evaluated the effects of hsa_circ_0001306 on HCC cell proliferation, invasion, cell cycle. Finally, we used an animal model to validate the experimental results. The expression of hsa_circ_0001306 was closely related to tumor size. Knockdown of hsa_circ_0001306 could downregulate F-box and WD repeat domain containing 7(FBXW7), a target of miR-527, thereby promoting HCC cell proliferation and invasion. Furthermore, hsa_circ_0001306 siRNA increased the multiplication rate of HCC tumors. Mechanistic studies indicated that hsa_circ_0001306 acts as a ceRNA for miR-527, which resulted in the reduction of its endogenous target, FBXW7. Hsa_circ_001306 is significantly downregulated in HCC, and the hsa_circ_0001306/miR-527/FBXW7 axis plays an important role in HCC progression.

摘要

肝细胞癌（HCC）是全球最常见的癌症类型之一。据报道，环状RNA（circRNAs）可调节包括HCC在内的多种癌症。本研究旨在探讨hsa_circ_0001306在HCC中的潜在作用。首先，通过高通量RNA测序鉴定出hsa_circ_0001306的表达下调，并通过qRT-PCR进一步验证。其次，我们评估了hsa_circ_0001306对HCC细胞增殖、侵袭和细胞周期的影响。最后，我们使用动物模型验证实验结果。hsa_circ_0001306的表达与肿瘤大小密切相关。敲低hsa_circ_0001306可下调miR-527的靶标含F-box和WD重复结构域7（FBXW7），从而促进HCC细胞增殖和侵袭。此外，hsa_circ_0001306 siRNA提高了HCC肿瘤的增殖率。机制研究表明，hsa_circ_0001306作为miR-527的竞争性内源RNA（ceRNA），导致其内源靶标FBXW7减少。Hsa_circ_001306在HCC中显著下调，且hsa_circ_0001306/miR-527/FBXW7轴在HCC进展中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/52280c383cce/jcav12p6531g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/5313640b83d1/jcav12p6531g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/86ddbcc512f7/jcav12p6531g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/21a62a21c231/jcav12p6531g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/2c0a89d15205/jcav12p6531g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/52280c383cce/jcav12p6531g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/5313640b83d1/jcav12p6531g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/200a0fc400f6/jcav12p6531g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/f3986b33bb42/jcav12p6531g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/2c0a89d15205/jcav12p6531g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3d6/8489137/52280c383cce/jcav12p6531g007.jpg

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