用大体积充填和常规生物活性复合材料修复的牙齿的牙髓内过氧化氢浓度。

Intrapulpal Concentration of Hydrogen Peroxide of Teeth Restored With Bulk Fill and Conventional Bioactive Composites.

出版信息

Oper Dent. 2021 May 1;46(3):E158-E170. doi: 10.2341/20-091-L.

Abstract

This study evaluated intrapulpal concentration and hydrogen peroxide (HP) penetration at the interface of teeth restored with bioactive composites, using conventional or bulk-fill composites. Cylindrical cavities were prepared on the buccal surface of bovine incisor crowns (n=20) and restored with: resin modified glass-ionomer (RMGI, Riva Light Cure, SDI), non-bioactive bulk-fill composite (FB, Filtek Bulk, 3M Oral Care), non-bioactive conventional composite (FZ, Filtek Z350, 3M Oral Care), bioactive bulk-fill composite (AC, Activa BioActive, Pulpedent), and bioactive conventional composite (BII, Beautifil II, Shofu). After 5,000 thermal cycles, restorations (n=10) were exposed to high (35% HP [4 applications of 8 min/session-4 sessions]) or low (9.5% HP [30 min/day-14 days]) concentration bleaching protocols. After the last bleaching application, the HP intrapulpal concentration was determined. Additional teeth were restored, pigmented with rhodamine B solution, and HP penetration around the interface was observed under laser scanning confocal fluorescence microscopy (LSCFM, n=3). The presence of gaps at the interface was observed on replicas of the cross-sectioned samples under scanning electron microscopy (SEM, n=5). Data were submitted to one-way (gap analysis) and twoway analysis of variance (ANOVA; HP intrapulpal concentration) and Tukey test (α=0.05). The LSCFM images were qualitatively analyzed. The restored teeth submitted to 35% HP presented higher HP intrapulpal concentration than teeth submitted to 9.5% HP (p<0.05). No differences in HP intrapulpal concentration was observed among groups (p>0.05) when exposed to 9.5% HP. Lower HP intrapulpal concentration was observed for teeth restored with RMGI exposed to HP 35%, when compared with teeth restored with nonbioactive conventional (FZ; p=0.004) and bulk-fill composites (FB; p=0.01). No gap formation was observed at the outer enamel adhesive interface for all restorative materials. LSCFM images showed that 35% HP promoted greater degradation of rhodamine B at the enamel, except for RMGI. In this context, RMGI promoted lower HP intrapulpal concentration than non-bioactive conventional and bulk-fill composites.

摘要

本研究评估了使用传统或块状充填复合材料修复的牙齿的牙髓内浓度和过氧化氢（HP）在牙本质界面的渗透性。在牛侧切牙冠的颊面制备圆柱形腔（n=20），并用以下材料修复：树脂改良型玻璃离子（RMGI，Riva Light Cure，SDI）、非生物活性块状充填复合材料（FB，Filtek Bulk，3M 口腔护理）、非生物活性传统复合材料（FZ，Filtek Z350，3M 口腔护理）、生物活性块状充填复合材料（AC，Activa BioActive，Pulpedent）和生物活性传统复合材料（BII，Beautifil II，Shofu）。经过 5000 次热循环后，对（n=10）个修复体进行高浓度（35% HP[4 次 8 分钟/次-4 次]）或低浓度（9.5% HP[30 分钟/天-14 天]）漂白方案处理。最后一次漂白应用后，测定牙髓内 HP 浓度。用罗丹明 B 溶液对其他牙齿进行着色，并用激光扫描共聚焦荧光显微镜（LSCFM，n=3）观察界面周围的 HP 渗透情况。在扫描电子显微镜（SEM，n=5）下观察到复制的横切片样本界面处存在间隙。数据采用单向（间隙分析）和双向方差分析（ANOVA；牙髓内 HP 浓度）和 Tukey 检验（α=0.05）进行处理。对 LSCFM 图像进行定性分析。用 35% HP 处理的修复牙的牙髓内 HP 浓度高于用 9.5% HP 处理的修复牙（p<0.05）。当用 9.5% HP 处理时，各组之间的牙髓内 HP 浓度没有差异（p>0.05）。用 35% HP 处理的 RMGI 修复牙的牙髓内 HP 浓度低于用非生物活性传统复合材料（FZ；p=0.004）和块状充填复合材料（FB；p=0.01）修复牙。所有修复材料的外釉质黏附界面均未见间隙形成。LSCFM 图像显示，除了 RMGI 外，35% HP 可促进罗丹明 B 在牙釉质中的更大降解。在这种情况下，RMGI 比非生物活性传统复合材料和块状充填复合材料可降低牙髓内 HP 浓度。