Gao Ke, Guo Zong-Hao, Xue Chen, Gao Wen-Hui, Liu Yuan, Jiang Li
College of Life Science, Huaibei Normal University, Huaibei 235000, Anhui, China.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Ying Yong Sheng Tai Xue Bao. 2021 Aug;32(8):2949-2957. doi: 10.13287/j.1001-9332.202108.033.
As a new soil amendment strategy, the effects of biochar application on soil microbial community have been reported in literature, but little information is available on the response of nitrogen-related microbial communities to biochar application in the reclaimed soil from coal-mining area. Through an outdoor pot experiment with reclaimed soil from coal-mining area of Huaibei, the effects of biochar and biochar compound fertilizer on nitrifier and denitrifier communities were investigated by real time PCR (qPCR) and terminal-restricted fragment length polymorphism (T-RFLP). There were five treatments: control (CK), NPK fertilizer (CF), biochar compound fertili-zer (BF), 2% biochar and NPK fertilizer (LB), 4% biochar and NPK fertilizer (HB). Results showed that compared with the CK, the treatments CF, BF, LB and HB significantly increased the abundance of ammonia oxidizing archaea (AOA), ammonia oxidizing bacteria (AOB), and genes of denitrifier. Compared with the CF treatment, BF, LB and HB significantly increased the abundance of AOB and genes by 42.9%-82.1% and 33.5%-62.7%, respectively. Results of redundancy analysis showed that soil organic carbon, pH, NH-N and available potassium significantly affected AOB community structure, while soil organic carbon concentration, pH and NO-N concentration significantly altered -denitrifier community structure. Therefore, the application of biochar and biochar compound fertilizer could improve soil quality of the reclaimed soil from coal-mining area, and increase the abundances of nitrifier and denitrifier and alter community structure of AOB and -denitrifier.
作为一种新的土壤改良策略，文献中已报道了生物炭施用对土壤微生物群落的影响，但关于矿区复垦土壤中氮相关微生物群落对生物炭施用的响应信息却很少。通过在淮北矿区复垦土壤上进行的室外盆栽试验，利用实时荧光定量PCR（qPCR）和末端限制性片段长度多态性（T-RFLP）技术研究了生物炭和生物炭复合肥对硝化细菌和反硝化细菌群落的影响。试验设置了5个处理：对照（CK）、氮磷钾复合肥（CF）、生物炭复合肥（BF）、2%生物炭与氮磷钾复合肥（LB）、4%生物炭与氮磷钾复合肥（HB）。结果表明，与CK相比，CF、BF、LB和HB处理显著增加了氨氧化古菌（AOA）、氨氧化细菌（AOB）的丰度以及反硝化细菌的基因丰度。与CF处理相比，BF、LB和HB处理显著增加了AOB的丰度，增幅分别为42.9% - 82.1%，同时反硝化细菌基因丰度增幅为33.5% - 62.7%。冗余分析结果表明，土壤有机碳、pH值、铵态氮和速效钾显著影响AOB群落结构，而土壤有机碳浓度、pH值和硝态氮浓度显著改变反硝化细菌群落结构。因此，生物炭和生物炭复合肥的施用可以改善矿区复垦土壤的质量，增加硝化细菌和反硝化细菌的丰度，并改变AOB和反硝化细菌的群落结构。
