Diaz L A, Roscoe J T, Eaglstein N F, Labib R S, Patel H P, Mutasim D F, Anhalt G J
Ann N Y Acad Sci. 1986;475:181-91. doi: 10.1111/j.1749-6632.1986.tb20867.x.
In 1957, Witbesky et al. put forward several criteria that ideally should be fulfilled in order to prove the pathogenic role of an autoantibody in a putative autoimmune disease. There can now be very little doubt of the autoimmune nature of this disease and of the primary role of autoantibodies in its pathogenesis. The evidence that supports the concept that pemphigus autoantibodies are of primary pathogenic importance in the disease is as follows: IgG class autoantibodies can be found both circulating in the serum and bound to the epithelial cell surfaces in and around lesions in patients with pemphigus. These autoantibodies, purified from the serum of pemphigus patients, can induce acantholytic lesions typical of pemphigus both in experimental animals (neonatal mice) and in human and murine epidermal cell cultures. These autoantibodies react with a specific antigen of the epidermal cell. This purified antigen has been used to immunize rabbits and the resulting antibodies are capable of inducing pemphigus-like lesions in neonatal mice.
1957年，维特贝斯基等人提出了几个标准，要证明自身抗体在假定的自身免疫性疾病中的致病作用，理想情况下应满足这些标准。现在，对于这种疾病的自身免疫性质以及自身抗体在其发病机制中的主要作用，几乎没有什么疑问了。支持天疱疮自身抗体在该疾病中具有主要致病重要性这一概念的证据如下：在天疱疮患者中，IgG类自身抗体既可以在血清中循环，也可以结合在病变部位及其周围的上皮细胞表面。从这些天疱疮患者血清中纯化得到的这些自身抗体，在实验动物（新生小鼠）以及人和鼠的表皮细胞培养物中，都能诱发典型的天疱疮棘层松解性病变。这些自身抗体与表皮细胞的一种特定抗原发生反应。这种纯化的抗原已被用于免疫兔子，所产生的抗体能够在新生小鼠中诱发天疱疮样病变。
