一种中药制剂对小鼠实验性脉络膜新生血管的治疗作用
Therapeutic effect of a traditional Chinese medicine formulation on experimental choroidal neovascularization in mouse.
作者信息
Zhang Yu-Fei, Jiang Chuan, Zhou Xiao-Hong, Wei Dong-Yu, Li Shao-Heng, Long Pan, Li Man-Hong, Zhang Zuo-Ming, Chen Tao, Du Hong-Jun
机构信息
Center of Clinical Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
The Air Force Hospital from Northern Theater PLA, Shenyang 110092, Liaoning Province, China.
出版信息
Int J Ophthalmol. 2021 Oct 18;14(10):1492-1500. doi: 10.18240/ijo.2021.10.04. eCollection 2021.
AIM
To investigate therapeutic effects of traditional Chinese medicine formulations, Hexuemingmu (HXMM) on laser-induced choroidal neovascularization (CNV) and follow-up effect in mice.
METHODS
C57BL/6 mice of 8-week-old were used and CNV was induced with 577 nm laser photocoagulation. Animals were randomly divided into groups and different doses of HXMM were administered daily. One, four, and eight weeks after the intervention, the electroretinogram (ERG), fundus fluorescence angiography, choroidal flat mount and immunofluorescence staining were preformed to evaluate the function and CNV formation. The expression levels of angiogenic proteins were determined by Western blotting and immunofluorescence staining. An analysis of variance and Kruskal-Wallis test were used to test the differences among the groups.
RESULTS
The results showed that HXMM effectively increased amplitude of ERG of mice (<0.05), alleviated fundus CNV leakage (<0.05), and reduced the area of neovascularization and the expression of angiogenic proteins (<0.05) after laser-induced CNV.
CONCLUSION
HXMM can protect the retinal function of mice after laser-induced CNV, and inhibit the CNV development.
目的
研究中药制剂和血明目（HXMM）对激光诱导的脉络膜新生血管（CNV）的治疗效果及在小鼠中的随访效果。
方法
使用8周龄的C57BL/6小鼠，通过577nm激光光凝诱导CNV。将动物随机分组，每天给予不同剂量的HXMM。干预后1周、4周和8周，进行视网膜电图（ERG）、眼底荧光血管造影、脉络膜平铺片和免疫荧光染色，以评估功能和CNV形成情况。通过蛋白质印迹法和免疫荧光染色测定血管生成蛋白的表达水平。采用方差分析和Kruskal-Wallis检验来检验组间差异。
结果
结果显示，HXMM可有效提高小鼠ERG的振幅（<0.05），减轻眼底CNV渗漏（<0.05），并减少激光诱导CNV后新生血管的面积和血管生成蛋白的表达（<0.05）。
结论
HXMM可保护激光诱导CNV后小鼠的视网膜功能，并抑制CNV的发展。
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