Department of Pharmacognosy, Faculty of Pharmacy Medical College, Jagiellonian University, Medyczna 9, 30-688, Kraków, Poland.
Department of Food Chemistry and Nutrition, Faculty of Pharmacy Medical College, Jagiellonian University, Medyczna 9, 30-688, Kraków, Poland.
Chem Biodivers. 2021 Nov;18(11):e2100552. doi: 10.1002/cbdv.202100552. Epub 2021 Oct 20.
In this study, two ursane-type triterpenes, i. e., ursolic acid (UA) and 3-O-β-acetoxyursolic acid (AUA), were isolated and quantified in Sorbus intermedia (EHRH.) PERS. fruits. UA and AUA levels differed slightly throughout fruit maturation, and both triterpenes showed similar dynamics of accumulation with the highest levels found in ripe fruits (up to 6.33±0.56 and 1.17±0.18 mg/g dw. of UA and AUA, respectively). The extracts derived from unripe fruits displayed significant cytotoxic effects against WM793, DU-145, PC3, 8505C, Caco2 and A549 cells, but no correlation between UA and AUA levels and cytotoxicity was found. On the other hand, extracts from mature fruits were not active towards almost all of the tested cell lines. The chemometric approach showed that the extracts derived from fruits harvested earlier clustered to form a clearly distanced group from extracts prepared from more-mature fruits. The extracts at higher concentrations formed separate groups, which indicated the concentration-dependent effect of these extracts on the cells.
在这项研究中，从欧李（EHRH.）果实中分离并定量了两种五环三萜，即熊果酸（UA）和 3-O-β-乙酰氧基熊果酸（AUA）。UA 和 AUA 的水平在果实成熟过程中略有差异，两种三萜的积累动态相似，在成熟果实中含量最高（分别高达 6.33±0.56 和 1.17±0.18mg/gdw UA 和 AUA）。未成熟果实提取物对 WM793、DU-145、PC3、8505C、Caco2 和 A549 细胞具有显著的细胞毒性作用，但未发现 UA 和 AUA 水平与细胞毒性之间存在相关性。另一方面，成熟果实的提取物对几乎所有测试的细胞系均无活性。化学计量学方法表明，收获较早的果实提取物与从较成熟果实中制备的提取物聚类形成了明显的分离组。在较高浓度下，提取物形成了单独的组，这表明这些提取物对细胞的浓度依赖性影响。
