Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú.
Universidad Peruana Unión, Escuela Profesional Medicina Humana, Laboratorio de Investigación en Biología Molecular, Lima, Perú.
Biomedica. 2021 Oct 15;41(Sp. 2):180-187. doi: 10.7705/biomedica.5720.
Introduction. The emergence of multiresistant enterobacteria producing extendedspectrum beta-lactamase (ESBL) in outpatients with urinary tract infections represents a public health problem in Perú. Objectives. To characterize multiresistant enterobacteria isolated from patients diagnosed with urinary tract infection in two Peruvian jungle departments using molecular techniques. Materials and methods. We conducted a descriptive, observational, and retrospective study of 61 urine culture isolates from two departments in the Peruvian jungle during 2017-2018. Resistance profiles were identified using the MicroScan™ automated system and a conventional polymerase chain reaction (PCR) was used for the detection of blaCTX-M, blaTEM and blaSHV genes. Results. The most common positive ESBL enterobacteria for each department were Escherichia coli in Madre de Dios (10/40; 25%) and Ucayali (16/21; 76.2%). Gene blaCTX-M was the most prevalent in both departments (25/61; 41%), followed by blaTEM (15/61; 24.6%), and blaSHV (10/61; 16.4%). As for the antimicrobial susceptibility profile, we detected resistance levels of 72.6% for ampicillin, 82.3% for cephalothin, and 88.7% for nitrofurantoin. Conclusions. BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4% and blaCTX-M was the most common gene.
简介。在秘鲁，门诊尿路感染患者中出现产超广谱β-内酰胺酶（ESBL）的多药耐药肠杆菌是一个公共卫生问题。目的。使用分子技术对秘鲁丛林地区两个部门的尿路感染患者分离的多药耐药肠杆菌进行特征描述。材料和方法。我们对 2017 年至 2018 年期间秘鲁丛林地区两个部门的 61 份尿液培养分离物进行了描述性、观察性和回顾性研究。使用 MicroScan™自动系统鉴定耐药谱，并用常规聚合酶链反应（PCR）检测 blaCTX-M、blaTEM 和 blaSHV 基因。结果。Madre de Dios（40 例中的 10 例；25%）和 Ucayali（21 例中的 16 例；76.2%）部门最常见的阳性 ESBL 肠杆菌分别为大肠埃希菌。在两个部门中，blaCTX-M 基因最为普遍（61 例中的 25 例；41%），其次是 blaTEM（61 例中的 15 例；24.6%）和 blaSHV（61 例中的 10 例；16.4%）。至于抗菌药物敏感性谱，我们检测到氨苄西林的耐药率为 72.6%，头孢噻吩为 82.3%，呋喃妥因为 88.7%。结论。两个部门产 BLEE 的多药耐药肠杆菌菌株的比例为 57.4%，其中 blaCTX-M 是最常见的基因。
