van Vorstenbosch C J, van Rossum-Kok C M, Colenbrander B, Wensing C J
J Embryol Exp Morphol. 1986 Jun;95:261-77.
Testes of foetal pigs between 26 to 35 days post coitum (p.c.) were investigated histochemically and ultrastructurally. Diaphorase and delta 5-3 beta-hydroxysteroid dehydrogenase activities were studied using, respectively, NADH and pregnenolone and dihydroxy androsterone as substrates. Ultrastructurally, attention was focused on the development of mesenchymal cells and on the sustentacular cells in the primitive sex cords in an attempt to detect the origin of Leydig cells. Histochemically there is a concentration of activity toward the interstitium with increasing age. Also the reactions increase in intensity. Ultrastructurally no evidence for Leydig cell development from Sertoli cells could be observed. Mesenchymal cells between the sex cords show a development toward Leydig cells. This is absent in mesenchymal cells in the future tunica albuginea. Before 30 days p.c. no 'true' Leydig cells can be observed morphologically. The role of the rough endoplasmic reticulum/mitochondrial complex, which is present in many mesenchymal and sustentacular cells, is discussed.
对交配后26至35天的胎猪睾丸进行了组织化学和超微结构研究。分别以NADH以及孕烯醇酮和二羟雄甾酮为底物，研究了黄递酶和δ5-3β-羟基类固醇脱氢酶的活性。在超微结构方面，重点关注间充质细胞和原始性索中支持细胞的发育，试图检测睾丸间质细胞的起源。组织化学研究表明，随着年龄增长，活性集中于间质，反应强度也增加。超微结构观察未发现支持细胞发育为睾丸间质细胞的证据。性索之间的间充质细胞呈现出向睾丸间质细胞发育的趋势，而未来白膜中的间充质细胞则没有这种现象。在交配后30天之前，在形态学上观察不到“真正的”睾丸间质细胞。文中还讨论了许多间充质细胞和支持细胞中存在的粗面内质网/线粒体复合体的作用。
