Arfuso Francesca, Giannetto Claudia, Interlandi Claudia, Giudice Elisabetta, Bruschetta Antongiulio, Panzera Maria Francesca, Piccione Giuseppe
Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, Messina, Italy.
Orthopedic Institute "Franco Scalabrino" of Messina, Via Consolare Pompea, Messina, Italy.
J Equine Vet Sci. 2021 Nov;106:103753. doi: 10.1016/j.jevs.2021.103753. Epub 2021 Aug 29.
A deep knowledge on metabolic response of athlete horse to exercise is of paramount importance for the development of optimal training programs to attain equilibrium between good performance and animal welfare. This study investigated the dynamic change of metabolic, clotting times and peripheral indices of central fatigue in horses competing in an official 44 km endurance race. From 6 horses blood samples were collected at rest (T), within 5 minutes from the end of race (T), after 30, 60 minutes after the end of race (T and T) to investigate the values of blood lactate, glucose, platelets (PLTs), serum leucine, isoleucine, valine, tryptophan, the ratio between tryptophan and branched-chain amino acids (BCAAs) levels (Try/BCAAs), total proteins, non-esterified fatty acids, plasma thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time and fibrinogen (Fb). Lactate, PLTs, non-esterified fatty acids, BCAAs and Try/BCAAs showed higher values at T Tand Tthan T(P < .0001); glucose showed lower values at T Tand Tthan T(P < .0001). Higher TT values were found at T Tand Tthan T and at Tthan T and T(P < .0001); Fb was higher at Tthan T T and T(P < .0001). This survey confirms that horses performing prolonged physical exercise experienced glycolysis, lipid mobilization and amino acid metabolism to provide substrates as fuel for muscle contraction.
深入了解运动马的代谢反应对于制定最佳训练计划以实现良好表现与动物福利之间的平衡至关重要。本研究调查了参加44公里官方耐力赛的马匹代谢、凝血时间和中枢疲劳外周指标的动态变化。从6匹马身上采集血液样本，分别在休息时（T0）、比赛结束后5分钟内（T1）、比赛结束后30分钟（T2）、60分钟（T3），以检测血乳酸、葡萄糖、血小板（PLTs）、血清亮氨酸、异亮氨酸、缬氨酸、色氨酸、色氨酸与支链氨基酸（BCAAs）水平之比（Try/BCAAs）、总蛋白、非酯化脂肪酸、血浆凝血酶时间（TT）、凝血酶原时间（PT）、活化部分凝血活酶时间和纤维蛋白原（Fb）的值。乳酸、PLTs、非酯化脂肪酸、BCAAs和Try/BCAAs在T1、T2和T3时的值高于T0（P < .0001）；葡萄糖在T1、T2和T3时的值低于T0（P < .0001）。TT值在T1、T2和T3时高于T0，且在T2时高于T1和T3（P < .0001）；Fb在T2时高于T0、T1和T3（P < .0001）。这项调查证实，进行长时间体育锻炼的马匹经历了糖酵解、脂质动员和氨基酸代谢，以提供底物作为肌肉收缩的燃料。
