Munoz-Rivas A, Specht C A, Drummond B J, Froeliger E, Novotny C P, Ullrich R C
Mol Gen Genet. 1986 Oct;205(1):103-6. doi: 10.1007/BF02428038.
Protoplasts of a Schizophyllum commune tryptophan auxotroph (trp1), deficient in indole-3-glycerol phosphate synthetase (IGPS), were transformed to trp+ with plasmid DNA containing the Schizophyllum TRP1 sequence. Efficiencies up to 30 transformants per microgram of plasmid DNA were obtained. Southern blots reveal that the transforming DNA is integrated in chromosomal DNA. The trp+ phenotype of transformants is stable in meiosis and mitosis. Transformants possess IGPS activity comparable to wild-type cells.
裂褶菌色氨酸营养缺陷型（trp1）的原生质体缺乏吲哚 - 3 - 甘油磷酸合成酶（IGPS），用含有裂褶菌TRP1序列的质粒DNA将其转化为trp +。每微克质粒DNA可获得高达30个转化体的转化效率。Southern杂交显示转化DNA整合到染色体DNA中。转化体的trp +表型在减数分裂和有丝分裂中是稳定的。转化体具有与野生型细胞相当的IGPS活性。
