Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture Rural Affairs, Guangzhou 510300, China; Key Laboratory of Fishery Ecology and Environment, Guangdong Province, Guangzhou 510300, China; South China Sea Fishers Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China; Scientific Observation and Research Field Station of Pearl River Estuary Ecosystem, Guangdong Province, Guangzhou, 510300, China.
South China Sea Fishers Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China.
Mar Genomics. 2022 Feb;61:100910. doi: 10.1016/j.margen.2021.100910. Epub 2021 Oct 18.
In this study, we generated the transcriptome of Muraenesox cinereus from four combined tissues (muscle, sexual, liver and heart) using high-seq sequencing technology. De novo assembly was performed using Trinity software and a total of 62,125,296 high-quality clean reads were assembled and clustered into 75,862 unigene with an N50 of 2034 nt. After annotation, 43,157 unigenes had significant hit in Nr database. And then, 24,510 unigenes were annotated into three GO categories: biological processes, cellular components, and molecular functions. Moreover, 33,032 unigenes were mapped 25 different clusters of eukaryotic proteins. Furthermore, we predicted the structure of all unigenes using HMMER and MISA software, respectively. The result showed that a total of 33,183 nucleotide sequences of coding regions (direction of the sequences is 5'- > 3') were confirmed to the protein database and a total of 29,487 simple sequence repeats (SSR) were identified. The whole transcriptome is an important foundation for future genomic research on the M. cinereus, it could provide comprehensively understanding and further characterizations of transcriptomes of non-model organisms.
在这项研究中，我们使用高通量测序技术从四种组合组织（肌肉、生殖腺、肝脏和心脏）中生成了灰海鳗的转录组。使用 Trinity 软件进行从头组装，共组装和聚类出 75862 个高质量的 unigene，N50 为 2034 nt。注释后，43157 个 unigene 在 Nr 数据库中有显著命中。然后，24510 个 unigene 被注释到三个 GO 类别：生物过程、细胞成分和分子功能。此外，33032 个 unigene 被映射到 25 个不同的真核蛋白簇。此外，我们分别使用 HMMER 和 MISA 软件预测了所有 unigene 的结构。结果表明，共有 33183 个编码区核苷酸序列（序列方向为 5' - > 3'）被确认为蛋白质数据库，共鉴定出 29487 个简单重复序列（SSR）。全转录组是未来灰海鳗基因组研究的重要基础，它可以为非模式生物的转录组提供全面的理解和进一步的特征描述。
