Eye Clinic Sulzbach, Knappschaft Hospital Saar, An der Klinik 10, 66280, Sulzbach, Germany.
Klaus Heimann Eye Research Institute (KHERI), Knappschaft Hospital Saar, Sulzbach, Germany.
Int Ophthalmol. 2022 Feb;42(2):401-409. doi: 10.1007/s10792-021-02056-w. Epub 2021 Oct 21.
To investigate the clinical outcome and complication rate of precut Descemet membrane endothelial keratoplasty (DMEK) in two different culture conditions, dextran-containing and dextran-free medium, and compare the results with the current standard DMEK procedure.
A prospective study of 32 eyes suffering from Fuchs endothelial dystrophy were scheduled for DMEK with a follow-up of one year. The eyes were divided into four subgroups. Group + D (n = 7) received a precut DMEK stored in dextran-containing transport medium, and Group - D (n = 9) received a precut DMEK without dextran-containing medium. The respective fellow eyes received a standard DMEK (S) (preparation directly prior to surgery) stored in dextran-containing medium (S-D + ; n = 7) or without (S-D-; n = 9).
Clinical outcome (visual acuity, endothelial cell count, central corneal thickness) and rebubbling rate were comparable for all four groups. None of the patients had a graft failure.
The preliminary data of the pilot study show that precut liquid-bubble DMEK leads to comparable clinical results regardless of dextran-containing or dextran-free organ culture medium and is further comparable to the standard DMEK procedure.
研究两种不同培养条件下（含葡聚糖和无葡聚糖培养基）前切割 Descemet 膜内皮角膜移植术（DMEK）的临床效果和并发症发生率，并与目前的标准 DMEK 手术进行比较。
对 32 只患有 Fuchs 内皮营养不良的眼睛进行前瞻性研究，计划进行 DMEK 手术，随访一年。这些眼睛被分为四组。+D 组（n=7）接受含葡聚糖的运输培养基中储存的前切割 DMEK，-D 组（n=9）接受不含葡聚糖的前切割 DMEK。各自的对侧眼接受含葡聚糖的标准 DMEK（S）（手术前直接准备）（S-D+；n=7）或不含葡聚糖（S-D-；n=9）。
所有四组的临床结果（视力、内皮细胞计数、中央角膜厚度）和再灌流率均相似。没有患者发生移植物失败。
初步研究数据表明，无论使用含葡聚糖还是无葡聚糖的器官培养培养基，前切割液泡 DMEK 均可产生相似的临床效果，并且与标准 DMEK 手术进一步相似。
