Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2022;2398:47-55. doi: 10.1007/978-1-0716-1912-4_4.
The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the genetic mechanisms that generate oscillations of gene expression over the course of the day. However, its use for the study of spatial regulation is often limited by resolution. Here, we describe a modified luciferase imaging method for the study of the Arabidopsis circadian clock across the plant at sub-tissue-level resolution.
拟南芥的生物钟是一个生成丰富时间和空间动态的基因网络的例子。生物发光成像已被证明是一种强大的方法，可以帮助剖析在一天的过程中产生基因表达振荡的遗传机制。然而，它在空间调控研究中的应用通常受到分辨率的限制。在这里，我们描述了一种改良的荧光素酶成像方法，用于在亚组织水平分辨率下研究拟南芥生物钟在整个植物中的情况。
