Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Germany.
Lab Anim. 2022 Feb;56(1):83-87. doi: 10.1177/00236772211051842. Epub 2021 Oct 21.
The introduction of the CRISPR-Cas9 system in 2013 has revolutionized experimental genetics in mice and rats. This commentary gives an overview on the use of CRISPR either for gene editing in the germline or for editing and beyond editing in somatic cells. Future perspectives are opened by emerging CRISPR technologies that could enable genome engineering at larger scale.
2013 年 CRISPR-Cas9 系统的引入彻底改变了小鼠和大鼠的实验遗传学。本评论概述了 CRISPR 在种系基因编辑或体细胞编辑及超越编辑中的应用。新兴的 CRISPR 技术为更大规模的基因组工程开辟了未来的前景。
