• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状CHFR通过miR-15a-5p/表皮生长因子受体轴调控人脑海微血管内皮细胞中氧化型低密度脂蛋白介导的细胞增殖、凋亡和内皮-间充质转化。

circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells.

作者信息

Wu Shanwu, Yang Sheng, Qu Hongyan

机构信息

Department of Neurosurgery, Sinopharm Dongfeng General Hospital, No. 16 Daling Road, Zhangwan District, Shiyan City, 442000, Hubei, China.

出版信息

Open Life Sci. 2021 Sep 29;16(1):1053-1063. doi: 10.1515/biol-2021-0082. eCollection 2021.

DOI:10.1515/biol-2021-0082
PMID:34676300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8483062/
Abstract

Oxidized low-density lipoprotein (ox-LDL) is a significant risk factor for various brain vascular diseases. Circular RNA (circRNA) is involved in the pathogenesis of brain vascular diseases. This study revealed the roles of circ_CHFR in ox-LDL-mediated cell proliferation, apoptosis, and endothelial-to-mesenchymal transition (EndoMT). Our results showed that circ_CHFR and EGFR expressions were dramatically upregulated, while miR-15a-5p expression was downregulated in ox-LDL-induced human brain microvessel endothelial cells (HBMECs) relative to control groups. circ_CHFR knockdown hindered the effects of ox-LDL exposure on cell proliferation, cell cycle, apoptosis, and EndoMT in HBMECs, whereas these impacts were abolished by miR-15a-5p inhibitor. In addition, circ_CHFR functioned as a sponge of miR-15a-5p and miR-15a-5p bound to EGFR. Thus, we concluded that circ_CHFR silencing hindered ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by downregulating EGFR expression through sponging miR-15a-5p in HBMECs. Our findings provide a new mechanism for studying circRNA-directed therapy in ox-LDL-induced human brain vascular diseases.

摘要

氧化型低密度脂蛋白（ox-LDL）是多种脑血管疾病的重要危险因素。环状RNA（circRNA）参与脑血管疾病的发病机制。本研究揭示了circ_CHFR在ox-LDL介导的细胞增殖、凋亡和内皮-间充质转化（EndoMT）中的作用。我们的结果表明，相对于对照组，在ox-LDL诱导的人脑微血管内皮细胞（HBMECs）中，circ_CHFR和表皮生长因子受体（EGFR）的表达显著上调，而miR-15a-5p的表达下调。circ_CHFR基因敲低阻碍了ox-LDL暴露对HBMECs细胞增殖、细胞周期、凋亡和EndoMT的影响，而这些影响被miR-15a-5p抑制剂消除。此外，circ_CHFR充当miR-15a-5p的海绵，且miR-15a-5p与EGFR结合。因此，我们得出结论，circ_CHFR沉默通过在HBMECs中充当miR-15a-5p的海绵来下调EGFR表达，从而阻碍ox-LDL介导的细胞增殖、凋亡和EndoMT。我们的研究结果为研究circRNA导向治疗ox-LDL诱导的人脑血管疾病提供了一种新机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/5ed450bb8571/j_biol-2021-0082-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/18dbdd477656/j_biol-2021-0082-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/38abb0e3b99b/j_biol-2021-0082-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/76d3ec6dface/j_biol-2021-0082-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/f9cd6e518bea/j_biol-2021-0082-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/fc848c833027/j_biol-2021-0082-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/a509880c737b/j_biol-2021-0082-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/5ed450bb8571/j_biol-2021-0082-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/18dbdd477656/j_biol-2021-0082-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/38abb0e3b99b/j_biol-2021-0082-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/76d3ec6dface/j_biol-2021-0082-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/f9cd6e518bea/j_biol-2021-0082-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/fc848c833027/j_biol-2021-0082-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/a509880c737b/j_biol-2021-0082-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c3c/8483062/5ed450bb8571/j_biol-2021-0082-fig007.jpg

相似文献

1
circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells.环状CHFR通过miR-15a-5p/表皮生长因子受体轴调控人脑海微血管内皮细胞中氧化型低密度脂蛋白介导的细胞增殖、凋亡和内皮-间充质转化。
Open Life Sci. 2021 Sep 29;16(1):1053-1063. doi: 10.1515/biol-2021-0082. eCollection 2021.
2
Circ-CHFR modulates the proliferation, migration, and invasion of ox-LDL-induced human aorta vascular smooth muscle cells through the miR-214-3p/PAPPA axis.环状 CHFR 通过 miR-214-3p/PAPPA 轴调节 ox-LDL 诱导的人主动脉血管平滑肌细胞的增殖、迁移和侵袭。
Clin Hemorheol Microcirc. 2022;80(4):399-412. doi: 10.3233/CH-211288.
3
Circular RNA circ_0124644 exacerbates the ox-LDL-induced endothelial injury in human vascular endothelial cells through regulating PAPP-A by acting as a sponge of miR-149-5p.环状 RNA circ_0124644 通过作为 miR-149-5p 的海绵体来调节 PAPP-A，从而加剧了 ox-LDL 诱导的人血管内皮细胞中的内皮损伤。
Mol Cell Biochem. 2020 Aug;471(1-2):51-61. doi: 10.1007/s11010-020-03764-0. Epub 2020 Jun 4.
4
Circ_CHFR expedites cell growth, migration and inflammation in ox-LDL-treated human vascular smooth muscle cells via the miR-214-3p/Wnt3/β-catenin pathway.Circ_CHFR 通过 miR-214-3p/Wnt3/β-catenin 通路促进 ox-LDL 处理的人血管平滑肌细胞的生长、迁移和炎症。
Eur Rev Med Pharmacol Sci. 2020 Mar;24(6):3282-3292. doi: 10.26355/eurrev_202003_20696.
5
Circular RNA circ_0029589 regulates proliferation, migration, invasion, and apoptosis in ox-LDL-stimulated VSMCs by regulating miR-424-5p/IGF2 axis.环状 RNA circ_0029589 通过调控 miR-424-5p/IGF2 轴调控 ox-LDL 刺激的血管平滑肌细胞增殖、迁移、侵袭和凋亡。
Vascul Pharmacol. 2020 Dec;135:106782. doi: 10.1016/j.vph.2020.106782. Epub 2020 Aug 27.
6
Circ_0003423 Alleviates ox-LDL-Induced Human Brain Microvascular Endothelial Cell Injury via the miR-589-5p/TET2 Network.环状 RNA 0003423 通过 miR-589-5p/TET2 网络减轻 ox-LDL 诱导的人脑血管内皮细胞损伤。
Neurochem Res. 2021 Nov;46(11):2885-2896. doi: 10.1007/s11064-021-03387-x. Epub 2021 Jul 5.
7
Circ_HECW2 functions as a miR-30e-5p sponge to regulate LPS-induced endothelial-mesenchymal transition by mediating NEGR1 expression.环状 RNA（circRNA）HECW2 通过作为 miR-30e-5p 的海绵来调控 NEGR1 的表达，从而调节脂多糖诱导的内皮-间质转化。
Brain Res. 2020 Dec 1;1748:147114. doi: 10.1016/j.brainres.2020.147114. Epub 2020 Sep 9.
8
Knockdown of circular RNA hsa_circ_0003204 inhibits oxidative stress and apoptosis through the miR-330-5p/Nod2 axis to ameliorate endothelial cell injury induced by low-density lipoprotein.环状RNA hsa_circ_0003204的敲低通过miR-330-5p/Nod2轴抑制氧化应激和细胞凋亡，以改善低密度脂蛋白诱导的内皮细胞损伤。
Cent Eur J Immunol. 2021;46(2):140-151. doi: 10.5114/ceji.2021.108174. Epub 2021 Aug 7.
9
MicroRNA-25-5p counteracts oxidized LDL-induced pathological changes by targeting neuronal growth regulator 1 (NEGR1) in human brain micro-vessel endothelial cells.microRNA-25-5p 通过靶向神经元生长调节因子 1（NEGR1）在人脑血管内皮细胞中拮抗氧化型低密度脂蛋白诱导的病理变化。
Biochimie. 2019 Oct;165:141-149. doi: 10.1016/j.biochi.2019.07.020. Epub 2019 Jul 28.
10
Circ_USP36/miR-182-5p/KLF5 axis regulates the ox-LDL-induced injury in human umbilical vein smooth muscle cells.环状USP36/miR-182-5p/KLF5轴调控氧化型低密度脂蛋白诱导的人脐静脉平滑肌细胞损伤。
Am J Transl Res. 2020 Dec 15;12(12):7855-7869. eCollection 2020.

引用本文的文献

1
Broader Perspective on Atherosclerosis-Selected Risk Factors, Biomarkers, and Therapeutic Approach.动脉粥样硬化的更广阔视角——选定的风险因素、生物标志物和治疗方法。
Int J Mol Sci. 2024 May 10;25(10):5212. doi: 10.3390/ijms25105212.
2
Role of Circular RNAs in Atherosclerosis through Regulation of Inflammation, Cell Proliferation, Migration, and Apoptosis: Focus on Atherosclerotic Cerebrovascular Disease.环状 RNA 在动脉粥样硬化中的作用通过调节炎症、细胞增殖、迁移和细胞凋亡实现：聚焦于动脉粥样硬化性脑血管病。
Medicina (Kaunas). 2023 Aug 14;59(8):1461. doi: 10.3390/medicina59081461.
3
Circular RNA as Therapeutic Targets in Atherosclerosis: Are We Running in Circles?

本文引用的文献

1
LncRNA BLACAT1/miR-519d-3p/CREB1 Axis Mediates Proliferation, Apoptosis, Migration, Invasion, and Drug-Resistance in Colorectal Cancer Progression.长链非编码RNA BLACAT1/微小RNA-519d-3p/环磷腺苷效应元件结合蛋白1轴在结直肠癌进展中介导增殖、凋亡、迁移、侵袭及耐药性
Cancer Manag Res. 2020 Dec 22;12:13137-13148. doi: 10.2147/CMAR.S274447. eCollection 2020.
2
Hsa_circ_0029589 knockdown inhibits the proliferation, migration and invasion of vascular smooth muscle cells via regulating miR-214-3p and STIM1.Hsa_circ_0029589 敲低通过调控 miR-214-3p 和 STIM1 抑制血管平滑肌细胞的增殖、迁移和侵袭。
Life Sci. 2020 Oct 15;259:118251. doi: 10.1016/j.lfs.2020.118251. Epub 2020 Aug 11.
3
环状RNA作为动脉粥样硬化的治疗靶点：我们在原地兜圈子吗？
J Clin Med. 2023 Jul 2;12(13):4446. doi: 10.3390/jcm12134446.
4
The uPA/uPAR System Orchestrates the Inflammatory Response, Vascular Homeostasis, and Immune System in Fibrosis Progression.uPA/uPAR 系统在纤维化进展中协调炎症反应、血管稳态和免疫系统。
Int J Mol Sci. 2023 Jan 16;24(2):1796. doi: 10.3390/ijms24021796.
circ_0007385 served as competing endogenous RNA for miR-519d-3p to suppress malignant behaviors and cisplatin resistance of non-small cell lung cancer cells.
circ_0007385 作为竞争性内源性 RNA，与 miR-519d-3p 结合，抑制非小细胞肺癌细胞的恶性行为和顺铂耐药性。
Thorac Cancer. 2020 Aug;11(8):2196-2208. doi: 10.1111/1759-7714.13527. Epub 2020 Jun 29.
4
Circular RNA circ_0003645 silencing alleviates inflammation and apoptosis via the NF-κB pathway in endothelial cells induced by oxLDL.环状 RNA circ_0003645 通过沉默减轻 oxLDL 诱导的内皮细胞中的炎症和细胞凋亡通过 NF-κB 通路。
Gene. 2020 Sep 10;755:144900. doi: 10.1016/j.gene.2020.144900. Epub 2020 Jun 15.
5
Inhibition of Brain Epidermal Growth Factor Receptor Activation: A Novel Target in Neurodegenerative Diseases and Brain Injuries.抑制脑表皮生长因子受体激活：神经退行性疾病和脑损伤的新靶点。
Mol Pharmacol. 2020 Jul;98(1):13-22. doi: 10.1124/mol.120.119909. Epub 2020 Apr 29.
6
MiR-21 suppresses ox-LDL-induced HUVECs apoptosis by targeting PDCD4.微小RNA-21通过靶向程序性细胞死亡蛋白4抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞凋亡。
Int J Clin Exp Pathol. 2017 Sep 1;10(9):10075-10084. eCollection 2017.
7
miR-15a-5p, miR-15b-5p, and miR-16-5p inhibit tumor progression by directly targeting MYCN in neuroblastoma.miR-15a-5p、miR-15b-5p 和 miR-16-5p 通过直接靶向神经母细胞瘤中的 MYCN 抑制肿瘤进展。
Mol Oncol. 2020 Jan;14(1):180-196. doi: 10.1002/1878-0261.12588. Epub 2019 Nov 29.
8
miR-17 regulates the proliferation and apoptosis of endothelial cells in coronary heart disease via targeting insulin-like-growth factor 1.miR-17 通过靶向胰岛素样生长因子 1 调节冠心病内皮细胞的增殖和凋亡。
Pathol Res Pract. 2019 Sep;215(9):152512. doi: 10.1016/j.prp.2019.152512. Epub 2019 Jun 20.
9
The role of circular RNA HECTD1 expression in disease risk, disease severity, inflammation, and recurrence of acute ischemic stroke.环状 RNA HECTD1 表达在急性缺血性脑卒中的疾病风险、疾病严重程度、炎症和复发中的作用。
J Clin Lab Anal. 2019 Sep;33(7):e22954. doi: 10.1002/jcla.22954. Epub 2019 Jul 1.
10
CircRNA‑0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR‑107.环状 RNA-0044073 在动脉粥样硬化中上调，并通过靶向 miR-107 增加细胞的增殖和侵袭。
Mol Med Rep. 2019 May;19(5):3923-3932. doi: 10.3892/mmr.2019.10011. Epub 2019 Mar 6.