He Lvqin, Yan Xuefeng, Dai Ke, Wen Xintian, Cao Sanjie, Huang Xiaobo, Wu Rui, Zhao Qin, Huang Yong, Yan Qigui, Ma Xiaoping, Han Xinfeng, Wen Yiping
Technology Department, Experimental Animal Center, Southwest Medical University, Luzhou, China.
Technology Department, Southwest Medical University, Luzhou, China.
Funct Integr Genomics. 2021 Nov;21(5-6):695-707. doi: 10.1007/s10142-021-00800-0. Epub 2021 Oct 21.
Haemophilus (Glaesserella) parasuis is a commensal bacterium that causes Glässer's disease (GD) in swine. As a global transcriptional factor, CheY regulates the expression of hundreds of genes in H. parasuis. In this study, we measured changes in gene expression at the whole transcriptome level using RNAseq. We identified 2058 co-expressed genes, and found 624 differentially expressed genes (q < 0.05) in ΔcheY and SC1401. Several important GO annotations and signaling pathways were identified. RNA-seq results were assembled according to the reference genome, compared with the annotated gene model, and 12 new transcriptional regions were found. Finally, q-PCR results validated the RNA-seq results with 8 randomly selected genes. The present study indicated that CheY is mainly involved in the regulation of ABC transport, oxidative phosphorylation, and β-Lactam resistance. We draw the regulatory network of CheY, which offers greater insight into the regulatory mechanism of CheY in H.parasuis.
副猪嗜血杆菌（格氏杆菌属）是一种共生细菌，可引起猪的格氏病（GD）。作为一种全局转录因子，CheY调节副猪嗜血杆菌中数百个基因的表达。在本研究中，我们使用RNAseq在全转录组水平上测量了基因表达的变化。我们鉴定出2058个共表达基因，并在ΔcheY和SC1401中发现了624个差异表达基因（q < 0.05）。确定了几个重要的GO注释和信号通路。RNA-seq结果根据参考基因组进行组装，与注释的基因模型进行比较，发现了12个新的转录区域。最后，q-PCR结果用8个随机选择的基因验证了RNA-seq结果。本研究表明，CheY主要参与ABC转运、氧化磷酸化和β-内酰胺抗性的调节。我们绘制了CheY的调控网络，这为深入了解CheY在副猪嗜血杆菌中的调控机制提供了更多信息。
