Department of Sericulture, College of Biological and Agricultural Engineering, Weifang University, Weifang, China.
Department of Sericulture, College of Forestry, Shandong Agricultural University, Taian, Shandong, China.
Arch Insect Biochem Physiol. 2021 Dec;108(4):e21848. doi: 10.1002/arch.21848. Epub 2021 Oct 22.
Bombyx mori nucleopolyhedrovirus (BmNPV) infection causes a series of physiological and pathological changes in Bombyx mori (B. mori). Here, a metabolomic study of the innate immunity organs including hemolymph, fat body, and midgut of the silkworm strain Dazao following BmNPV challenge was conducted to reveal the metabolic variations in B. mori. Compared to the control, 4964 and 4942 features with 4077 and 4327 high-quality features were generated under positive and negative modes, respectively, from BmNPV-infected larvae. The principal component analysis and supervised learning method using partial least squares discrimination analysis demonstrated good analytical stability and experimental reproducibility of the metabolic profiles. Based on database annotations, a total of 296, 108, and 215 differential expressed metabolites (DEMs) were identified from BmNPV-infected group of hemolymph, fat body, and midgut, respectively, which were all mainly grouped into carboxylic acids and derivatives, fatty acyls, and glycerophospholipids. Kyoto Encyclopedia of Genes and Genomes Database enrichment analysis of the DEMs showed that amino acid metabolism was increased at 24 h after BmNPV infection. BmNPV induction was adopted to significantly alter a series of immune-related pathways including phospholipase D signaling pathway, FoxO signaling pathway, metabolism of xenobiotics by cytochrome P450, melanogenesis, membrane transport, carbohydrate metabolism, and lipid metabolism. The different levels of expression of several DEMs including l-glutamate, naphthalene, 3-succinoylpyridine 1-acyl-sn-glycerol 3-phosphate, and l-tyrosine which were involved in those pathways exhibited the immune responses of B. mori to BmNPV infection. Our findings are valuable for a better understanding of the antiviral mechanism of B. mori underlying the interaction between the silkworm and BmNPV.
家蚕核型多角体病毒（BmNPV）感染会引起家蚕（B. mori）一系列生理和病理变化。在这里，我们对家蚕感染 BmNPV 后先天免疫器官（包括血液、脂肪体和中肠）进行了代谢组学研究，以揭示家蚕的代谢变化。与对照组相比，正、负模式下从 BmNPV 感染幼虫中分别生成了 4964 个和 4942 个特征，其中有 4077 个和 4327 个高质量特征。主成分分析和偏最小二乘判别分析的监督学习方法表明代谢谱具有良好的分析稳定性和实验可重复性。基于数据库注释，在血液、脂肪体和中肠的 BmNPV 感染组中分别鉴定出 296、108 和 215 个差异表达代谢物（DEMs），它们主要分为羧酸及其衍生物、脂肪酸和甘油磷脂。DEMs 的京都基因与基因组百科全书数据库富集分析表明，BmNPV 感染后 24 小时氨基酸代谢增加。BmNPV 诱导显著改变了一系列与免疫相关的途径，包括磷酸二酯酶信号通路、FoxO 信号通路、细胞色素 P450 代谢外来化合物、黑色素生成、膜转运、碳水化合物代谢和脂质代谢。涉及这些途径的几种 DEMs，包括 l-谷氨酸、萘、3-琥珀酰吡啶 1-酰基-sn-甘油-3-磷酸和 l-酪氨酸的表达水平，均表现出家蚕对 BmNPV 感染的免疫反应。我们的研究结果为更好地理解家蚕与 BmNPV 相互作用下的抗病毒机制提供了有价值的信息。
