Wang Yuanyu, Zhang Ruihua, Zhang Qiang, Cao Chengxi, Fan Liuyin, Liu Weiwen
School of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai,200240, China.
Student Innovation Center, Shanghai Jiao Tong University, Shanghai, 200240, China.
Se Pu. 2021 Nov;39(11):1151-1156. doi: 10.3724/SP.J.1123.2021.04030.
The existing miniature capillary electrophoresis (CE) devices use a tablet or a computer for data processing and analysis, which hinders their portability. In order to solve this problem, a smartphone-based CE device was proposed, which allowed for real portable quantitative analysis. The device integrated the functions of capacitively coupled contactless conductivity detection (CD) and Bluetooth communication. Furthermore, a Kotlin language-based application with a user-friendly interface was developed. The application could not only control the electrophoresis run in the CE device but also receive the data from the CD detector in real time, display the electrophoretogram, and process the data. The peak areas could be calculated automatically on the smartphone, and the migration time could be obtained. The size of the developed device was 20 cm×20 cm×15 cm, and its weight was 2 kg. Quaternary ammonium salts (QAs) in disinfectors (dodecyl dimethyl benzyl ammonium bromide (DDBAB) and dodecyl trimethyl ammonium bromide (DTAB)) were used as the analytes to verify the performance of the developed device. The experimental data showed that the linear ranges of DDBAB and DTAB were from 20 to 1000 and from 30 to 1000 μmol/L, respectively. The correlation coefficients () of DDBAB and DTAB were 0.9995 and 0.9989, respectively, indicating good linearity between the peak area and concentration. The limits of detection (LODs) of DDBAB and DTAB were 10 and 13 μmol/L, respectively. The intra-day relative standard deviations (RSDs, =3) of DDBAB and DTAB were 1.9% and 2.7% respectively, revealing good repeatability. In addition, a mixture of DDBAB and DTAB was tested. Two QAs were separated within 8 min, showing good selectivity. Finally, QAs in a bromo geramine disinfector used in the field were tested to further validate the performance of the designed device. The recoveries of DDBAB and DTAB were 100.5%-101.5% and 96.2%-99.3%, respectively, indicating good accuracy. The developed device has the advantages of good linearity, low LOD, good repeatability, high accuracy, and real portability, and it can be used for the quantitative detection of QAs in disinfectors.
现有的微型毛细管电泳（CE）设备使用平板电脑或计算机进行数据处理和分析，这阻碍了它们的便携性。为了解决这个问题，人们提出了一种基于智能手机的CE设备，它可以实现真正的便携式定量分析。该设备集成了电容耦合非接触式电导检测（CD）和蓝牙通信功能。此外，还开发了一个具有用户友好界面的基于Kotlin语言的应用程序。该应用程序不仅可以控制CE设备中的电泳运行，还可以实时接收来自CD检测器的数据，显示电泳图并处理数据。可以在智能手机上自动计算峰面积，并获得迁移时间。所开发设备的尺寸为20 cm×20 cm×15 cm，重量为2 kg。使用消毒剂中的季铵盐（QAs）（十二烷基二甲基苄基溴化铵（DDBAB）和十二烷基三甲基溴化铵（DTAB））作为分析物来验证所开发设备的性能。实验数据表明，DDBAB和DTAB的线性范围分别为20至1000和30至1000 μmol/L。DDBAB和DTAB的相关系数（）分别为0.9995和0.9989，表明峰面积与浓度之间具有良好的线性关系。DDBAB和DTAB的检测限（LOD）分别为10和13 μmol/L。DDBAB和DTAB的日内相对标准偏差（RSDs，=3）分别为1.9%和2.7%，显示出良好的重复性。此外，还测试了DDBAB和DTAB的混合物。两种QAs在8分钟内分离，显示出良好的选择性。最后，对现场使用的溴化新洁尔灭消毒剂中的QAs进行了测试，以进一步验证所设计设备的性能。DDBAB和DTAB的回收率分别为100.5%-101.5%和96.2%-99.3%，表明准确性良好。所开发的设备具有线性良好、检测限低、重复性好、准确性高和真正便携的优点，可用于消毒剂中QAs的定量检测。
