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基于智能手机的化学发光折纸微流控分析装置用于快速评估血糖水平

Smartphone-Based Chemiluminescent Origami µPAD for the Rapid Assessment of Glucose Blood Levels.

作者信息

Calabria Donato, Zangheri Martina, Trozzi Ilaria, Lazzarini Elisa, Pace Andrea, Mirasoli Mara, Guardigli Massimo

机构信息

Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum-University of Bologna, Via Francesco Selmi 2, I-40126 Bologna, Italy.

Interdepartmental Centre for Industrial Agrofood Research (CIRI AGRO), Alma Mater Studiorum-University of Bologna, Via Quinto Bucci 336, I-47521 Cesena, Italy.

出版信息

Biosensors (Basel). 2021 Oct 9;11(10):381. doi: 10.3390/bios11100381.

DOI:10.3390/bios11100381
PMID:34677337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8533763/
Abstract

Microfluidic paper analytical devices (µPADs) represent one of the most appealing trends in the development of simple and inexpensive analytical systems for diagnostic applications at the point of care (POC). Herein, we describe a smartphone-based origami µPAD for the quantitative determination of glucose in blood samples based on the glucose oxidase-catalyzed oxidation of glucose leading to hydrogen peroxide, which is then detected by means of the luminol/hexacyanoferrate(III) chemiluminescent (CL) system. By exploiting the foldable µPAD format, a two-step analytical procedure has been implemented. First, the diluted blood sample was added, and hydrogen peroxide was accumulated, then the biosensor was folded, and a transport buffer was added to bring hydrogen peroxide in contact with CL reagents, thus promoting the CL reaction. To enable POC applicability, the reagents required for the assay were preloaded in the µPAD so that no chemicals handling was required, and a 3D-printed portable device was developed for measuring the CL emission using the smartphone's CMOS camera. The µPAD was stable for 30-day storage at room temperature and the assay, displaying a limit of detection of 10 µmol L, proved able to identify both hypoglycemic and hyperglycemic blood samples in less than 20 min.

摘要

微流控纸基分析装置（µPADs）是即时检测（POC）诊断应用中简单且廉价分析系统开发领域最具吸引力的发展趋势之一。在此，我们描述了一种基于智能手机的折纸µPAD，用于定量测定血样中的葡萄糖。该方法基于葡萄糖氧化酶催化葡萄糖氧化生成过氧化氢，然后通过鲁米诺/铁氰化铁（III）化学发光（CL）系统进行检测。通过采用可折叠的µPAD形式，实施了两步分析程序。首先，加入稀释的血样并积累过氧化氢，然后折叠生物传感器，并加入传输缓冲液使过氧化氢与CL试剂接触，从而促进CL反应。为实现POC适用性，测定所需的试剂预先加载在µPAD中，因此无需进行化学试剂处理，并且开发了一种3D打印的便携式装置，用于使用智能手机的CMOS相机测量CL发射。该µPAD在室温下储存30天稳定，该测定法的检测限为10 µmol/L，能够在不到20分钟内识别低血糖和高血糖血样。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/8e865d82e80b/biosensors-11-00381-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/ea003efadb0a/biosensors-11-00381-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/0c1588c96d5b/biosensors-11-00381-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/cac66e9f610b/biosensors-11-00381-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/820e635d0874/biosensors-11-00381-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/8e865d82e80b/biosensors-11-00381-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/ea003efadb0a/biosensors-11-00381-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/529e83fe9363/biosensors-11-00381-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/83bddcef2c86/biosensors-11-00381-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/82ac640c986c/biosensors-11-00381-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/0c1588c96d5b/biosensors-11-00381-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/cac66e9f610b/biosensors-11-00381-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/820e635d0874/biosensors-11-00381-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b5/8533763/8e865d82e80b/biosensors-11-00381-g008.jpg

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