3M™ Petrifilm™ 大肠菌群计数平板验证用于瓶装水中大肠菌群的计数：AOAC 经性能测试的方法 SM 082101。

Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested MethodSM 082101.

机构信息

3M Food Safety Department, 3M Center, Bldg. 260-6B-01, St. Paul, MN 55144-1000, USA and.

Research and Development, SGS Vanguard Sciences, 224 North Derby Lane, North Sioux City, SD 57049, USA.

出版信息

J AOAC Int. 2022 Apr 27;105(3):866-875. doi: 10.1093/jaoacint/qsab137.

DOI:10.1093/jaoacint/qsab137

PMID:34677581

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9046964/

Abstract

BACKGROUND

The 3M™ Petrifilm™ Coliform Count (CC) Plate is a sample-ready-culture medium system which contains modified Violet Red Bile nutrients, a cold-water-soluble gelling agent, and a tetrazolium indicator that facilitates colony enumeration.

OBJECTIVE

To validate the 3M Petrifilm CC Plate method for bottled water through the AOAC® Performance Tested Method(s)SM program.

METHODS

The performance of the 3M Petrifilm CC Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 4, Section III.D for the enumeration of coliforms in bottled water. Matrix data were normalized by log10 transformation and performance indicators included repeatability and difference of means with 90 and 95% confidence intervals. Inclusivity, exclusivity, robustness, and product consistency and stability were evaluated.

RESULTS

This study demonstrated that the 3M Petrifilm CC Plate method detects and enumerates coliforms from bottled water. The average log10 counts of the 3M Petrifilm CC Plate method were equivalent to or better than the average log10 counts of the reference method. Results from inclusivity and exclusivity studies demonstrated that the 3M Petrifilm CC Plate method differentiated coliforms from non-coliform strains. In product consistency, stability, and robustness testing, different lots of 3M Petrifilm CC Plates and small deviations in incubation time and temperature did not affect test results.

CONCLUSION

The 3M Petrifilm CC Plate method is an accurate and robust method for the enumeration of coliforms in bottled water.

HIGHLIGHTS

The 3M Petrifilm CC Plate allows for detection of confirmed coliforms within 24 h. Up to 20 sample-ready plates can be stacked during incubation, providing flexibility and saving space.

摘要

背景

3M™ Petrifilm™ 大肠菌群计数（CC）平板是一种样本准备好的培养物系统，其中包含改良的紫色红胆汁营养物、冷水可溶性凝胶剂和四唑指示剂，有助于菌落计数。

目的

通过 AOAC® 性能测试方法（SM）计划验证 3M Petrifilm CC 平板法在瓶装水中的应用。

方法

将 3M Petrifilm CC 平板的性能与美国食品和药物管理局细菌分析手册第 4 章第 3 节 D 部分进行比较，用于瓶装水中大肠菌群的计数。通过对数 10 转换对基质数据进行归一化，并评估性能指标，包括 90%和 95%置信区间的重复性和平均值差异。还评估了包容性、排他性、稳健性以及产品一致性和稳定性。

结果

本研究表明，3M Petrifilm CC 平板法可检测和计数瓶装水中的大肠菌群。3M Petrifilm CC 平板法的平均 log10 计数与参考方法的平均 log10 计数相当或更好。包容性和排他性研究的结果表明，3M Petrifilm CC 平板法可区分大肠菌群和非大肠菌群菌株。在产品一致性、稳定性和稳健性测试中，不同批次的 3M Petrifilm CC 平板和孵育时间和温度的微小偏差不会影响测试结果。