3M™ Petrifilm 酵母菌和霉菌计数平板用于测定干燥大麻花中的酵母菌和霉菌：AOAC 官方方法 997.02。

3M™ Petrifilm Yeast and Mold Count Plate for the Enumeration of Yeasts and Molds in Dried Cannabis Flower: AOAC Official MethodSM 997.02.

机构信息

3M Food Safety Department, 3M Center, Bldg. 260-6B-01, St. Paul, MN 55144-1000, USA.

出版信息

J AOAC Int. 2023 Mar 1;106(2):412-419. doi: 10.1093/jaoacint/qsac114.

DOI:10.1093/jaoacint/qsac114

PMID:36171644

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9978580/

Abstract

BACKGROUND

The 3M™ Petrifilm™ Yeast and Mold (YM) Count Plate is a sample-ready culture medium system that contains nutrients supplemented with antibiotics, a cold-water-soluble gelling agent, and an indicator system that facilitates yeast and mold enumeration.

OBJECTIVE

The 3M Petrifilm YM Plate was validated for enumeration of yeast and mold in dried cannabis flower through the AOAC Emergency Response Validation process.

METHODS

The performance of the 3M Petrifilm YM Plate was compared to that of Dichloran Rose Bengal Chloramphenicol (DRBC) agar. Matrix data were normalized by log10 transformation, and performance indicators included repeatability, difference of means, and inclusivity/exclusivity.

RESULTS

These studies demonstrated that the 3M Petrifilm YM Plate method detects and enumerates yeasts and molds from cannabis flower at low, medium, and high contamination levels, and the average log counts at 20-25°C for 5 days were equivalent to the average log counts of the DRBC reference method. In strain studies, 59 out of 60 yeasts and molds produced typical colony morphology on 3M Petrifilm YM Plates. Of the nontarget bacterial strains tested, 38 out of 39 strains were not detected on 3M Petrifilm YM Plates.

CONCLUSIONS

The 3M Petrifilm YM Plate is a reliable method for the enumeration of live yeast and mold in dried cannabis flower.

HIGHLIGHTS

The 3M Petrifilm YM Plate allows for detection of yeast and mold within 5 days of incubation. The sample-ready plates can be incubated at 20-25°C and can be stacked up to 20 plates, thus providing user flexibility and saving incubator space.

摘要

背景

3M™ Petrifilm™ 酵母和霉菌（YM）计数平板是一种样本准备好的培养介质系统，其中含有营养物质、抗生素、冷水可溶性凝胶剂和一个指示系统，有助于酵母和霉菌的计数。

目的

通过 AOAC 紧急响应验证程序，对 3M Petrifilm YM 平板用于干燥大麻花中酵母和霉菌的计数进行验证。

方法

将 3M Petrifilm YM 平板的性能与 Dichloran Rose Bengal Chloramphenicol（DRBC）琼脂进行比较。通过对数转换对基质数据进行归一化，性能指标包括重复性、均值差异和包容性/排他性。

结果

这些研究表明，3M Petrifilm YM 平板法可检测和计数大麻花中低、中和高污染水平的酵母和霉菌，在 20-25°C 下培养 5 天的平均对数计数与 DRBC 参考方法的平均对数计数相当。在菌株研究中，60 株酵母和霉菌中有 59 株在 3M Petrifilm YM 平板上产生典型的菌落形态。在所测试的非目标细菌菌株中，有 39 株中的 38 株未在 3M Petrifilm YM 平板上检测到。