Nath Durbba, Chakraborty Supriyo
Department of Biotechnology, Assam University, Silchar, 788011, Assam, India.
J Appl Genet. 2022 Feb;63(1):169-182. doi: 10.1007/s13353-021-00666-9. Epub 2021 Oct 22.
The grievous adversity regarding Mycobacterium avium is its ubiquitous nature. Isolation of the bacteria from drinking water, house dust, and soil, etc., is an alarming issue for the scientific community. The microRNAs are the molecular influencers of gene expression that act during the process of post transcription. A few reports claimed the existence of microRNAs or microRNA-like molecules in the prokaryotic species. Biogenesis of bacterial miRNAs requires their transport into the host cell. Subsequently, the host-encoded enzymes are exerted for the formation of bacterial mature miRNAs and their regulation. In our study, the screening of complete genome of Mycobacterium avium revealed six putative precursor microRNA sequences bearing typical secondary structures. The mature microRNAs were predicted in both arms of the secondary structures. A total of 12 possible mature microRNAs were identified in this study. The likely targets of the predicted mature miRNAs were searched in human 3' UTR. In the human transcriptome, 193 genes were possibly targeted by 12 mature miRNAs of Mycobacterium avium. The essential functionalities of the target genes included signal transduction, immune system, DNA binding, and response to stress.
鸟分枝杆菌面临的严重困境在于其无处不在的特性。从饮用水、室内灰尘和土壤等中分离出这种细菌，对科学界来说是一个令人担忧的问题。微小RNA是在转录后过程中发挥作用的基因表达的分子影响因子。一些报告称原核生物物种中存在微小RNA或微小RNA样分子。细菌微小RNA的生物合成需要将它们转运到宿主细胞中。随后，宿主编码的酶用于形成细菌成熟微小RNA及其调控。在我们的研究中，对鸟分枝杆菌全基因组的筛选揭示了六个具有典型二级结构的假定前体微小RNA序列。在二级结构的两个臂中都预测到了成熟微小RNA。本研究共鉴定出12种可能的成熟微小RNA。在人类3'非翻译区中搜索预测的成熟微小RNA的可能靶标。在人类转录组中，鸟分枝杆菌的12种成熟微小RNA可能靶向193个基因。靶基因的基本功能包括信号转导、免疫系统、DNA结合和应激反应。
