Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Pathol Res Pract. 2021 Nov;227:153645. doi: 10.1016/j.prp.2021.153645. Epub 2021 Oct 14.
Breast cancer is the most common form of cancer in women around the world. The molecular mechanisms of this heterogeneous disease have been extensively investigated; but yet; It requires a lot of sensitive and specific markers for prognosis and early detection approaches. Non-protein coding RNAs known as lncRNAs have been reported in tumorigenic involvement so they can be used for therapeutic purposes. In the present study, the expression levels of CCAT1, PDCD4, PDCD4-AS1, and MEG3 LncRNA in adjacent tumor and breast tissue in 88 Iranian patients were evaluated by quantitative real-time PCR. CCAT1 was significantly expressed and PDCD4-AS1 decreased in tumor samples, PDCD4 and PDCD4-AS1 showed a positive correlation with each other, higher levels of PDCD4-AS1 were associated with better survival, tumor samples showed lower levels of PDCD4 in Showed comparisons with normal tissue. Our findings suggest that lncRNAs play an important role in controlling gene expression after transcription of major tumor suppressors or carcinogenic genes, leading to the development of triple-negative breast cancer (TNBC). In conclusion, this study investigated the prognostic role of lncRNA in breast cancer patients.
乳腺癌是全世界女性最常见的癌症形式。这种异质性疾病的分子机制已经得到了广泛的研究；但是，仍然需要大量敏感和特异的标志物用于预后和早期检测方法。非蛋白编码 RNA（lncRNA）已被报道参与肿瘤发生，因此可用于治疗目的。在本研究中，通过定量实时 PCR 评估了 88 例伊朗患者的相邻肿瘤和乳腺组织中 CCAT1、PDCD4、PDCD4-AS1 和 MEG3 lncRNA 的表达水平。CCAT1 在肿瘤样本中表达显著上调，PDCD4-AS1 下调，PDCD4 和 PDCD4-AS1 相互呈正相关，较高水平的 PDCD4-AS1 与更好的生存相关，肿瘤样本中 PDCD4 的表达水平较正常组织低。我们的研究结果表明，lncRNA 在转录后主要肿瘤抑制基因或致癌基因的基因表达调控中发挥重要作用，导致三阴性乳腺癌（TNBC）的发生。总之，本研究探讨了 lncRNA 在乳腺癌患者中的预后作用。
