Lee Kyungjong, Oh Mijung, Lee Kyo-Sun, Cha Yoon Jin, Chang Yoon Soo
Samsung Medical Center, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Sungkyunkwan University School of Medicine, Seoul 06351, Korea.
Medical Research Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea.
Diagnostics (Basel). 2021 Oct 3;11(10):1830. doi: 10.3390/diagnostics11101830.
Methionyl-tRNA synthetase (MARS) and A variant of Aminoacyl-tRNA synthetase interacting multifunctional protein 2 (AIMP2) with an exon 2 deletion (AIMP2-DX2) are known to be overexpressed in lung cancer. However, their role as diagnostic markers in lung cancer has not been well established. Thus, we evaluated their diagnostic performance in brushed cells obtained from nodular lung lesions suspected of lung cancer.
Samples obtained by radial endobronchial ultrasound-guided brushing were processed for cytological examination with Papanicolaou (Pap) staining. Then, double IF staining with MARS and AIMP2-DX2 antibodies was measured in the cytology samples for peripheral lung nodules. The diagnostic performance was compared against biomarkers.
MARS IF staining was the only independent staining method used for the prediction of malignant cells. The area under the curve (AUC) of conventional cytology, MARS IF, and MARS IF plus cytology was 0.64, 0.68, and 0.69, respectively. The diagnostic accuracy was increased in MARS IF plus conventional cytology compared with cytology alone (71% vs. 47%).
The combination of MARS staining with conventional cytology showed increases in the diagnostic accuracy for diagnosing lung nodules suspected of lung cancer on chest-computed tomography scans.
已知甲硫氨酰 - tRNA合成酶（MARS）和氨基酰 - tRNA合成酶相互作用多功能蛋白2的一种外显子2缺失变体（AIMP2 - DX2）在肺癌中过表达。然而，它们作为肺癌诊断标志物的作用尚未得到充分确立。因此，我们评估了它们在从疑似肺癌的肺部结节刷取细胞中的诊断性能。
对经径向支气管内超声引导刷取获得的样本进行巴氏（Pap）染色的细胞学检查。然后，在周围型肺结节的细胞学样本中用MARS和AIMP2 - DX2抗体进行双重免疫荧光（IF）染色。将诊断性能与生物标志物进行比较。
MARS免疫荧光染色是用于预测恶性细胞的唯一独立染色方法。传统细胞学、MARS免疫荧光以及MARS免疫荧光加细胞学的曲线下面积（AUC）分别为0.64、0.68和0.69。与单独细胞学检查相比，MARS免疫荧光加传统细胞学的诊断准确性有所提高（71%对47%）。
MARS染色与传统细胞学相结合，提高了在胸部计算机断层扫描中诊断疑似肺癌的肺部结节的诊断准确性。
