Pawluczuk Elżbieta, Łukaszewicz-Zając Marta, Gryko Mariusz, Kulczyńska-Przybik Agnieszka, Mroczko Barbara
Department of Neurodegeneration Diagnostics, Medical University of Bialystok, 15-269 Bialystok, Poland.
Department of Biochemical Diagnostics, Medical University of Bialystok, 15-269 Bialystok, Poland.
Cancers (Basel). 2021 Oct 15;13(20):5186. doi: 10.3390/cancers13205186.
Gastric cancer (GC) is the second leading cause of cancer-related deaths worldwide. This malignancy is usually diagnosed at an advanced stage. Therefore, novel biomarkers useful in the early detection of GC are sorely needed. Some authors suggest the role of chemokines and their specific receptors in GC pathogenesis. The aim of the study was to investigate whether serum CXCL8 and its receptor (CXCR2) might be considered as potential candidates for biomarkers in the diagnosis and prognosis of GC. The study included 98 subjects: 64 GC patients and 34 healthy volunteers. CXCL8 and CXCR2 concentrations were assessed by the enzyme-linked immunosorbent assay (ELISA) method. Serum CXCL8 and CXCR2 concentrations were significantly higher in GC patients than in healthy controls, similar to the well-established tumor marker (CA19-9) and marker of inflammation (CRP). Diagnostic sensitivity of CXCL8 was the highest among all proteins tested and increased for the combined assessment with CA19-9. The area under the ROC curve for CXCL8 was higher than those for CXCR2 and classical tumor markers. Serum CXCL8 levels were indicated as a significant risk factor of GC occurrence. Our findings suggest that serum CXCL8 is a promising candidate for a biomarker in GC diagnosis and might be used as a significant predictor of GC risk.
胃癌（GC）是全球癌症相关死亡的第二大主要原因。这种恶性肿瘤通常在晚期被诊断出来。因此，迫切需要有助于早期检测胃癌的新型生物标志物。一些作者提出趋化因子及其特异性受体在胃癌发病机制中的作用。本研究的目的是调查血清CXCL8及其受体（CXCR2）是否可被视为胃癌诊断和预后生物标志物的潜在候选者。该研究包括98名受试者：64名胃癌患者和34名健康志愿者。采用酶联免疫吸附测定（ELISA）法评估CXCL8和CXCR2的浓度。胃癌患者血清CXCL8和CXCR2浓度显著高于健康对照组，与公认的肿瘤标志物（CA19-9）和炎症标志物（CRP）相似。CXCL8的诊断敏感性在所有测试蛋白中最高，与CA19-9联合评估时增加。CXCL8的ROC曲线下面积高于CXCR2和经典肿瘤标志物。血清CXCL8水平被表明是胃癌发生的一个重要危险因素。我们的研究结果表明，血清CXCL8是胃癌诊断中一种有前景的生物标志物候选者，并且可能用作胃癌风险的重要预测指标。
