Chang Yanpeng, Zhang Yunhui, Geng Zichen, Yao Shuangyan, Zhao Wenli, Yin Xinming, An Shiheng
State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Insects. 2021 Sep 30;12(10):889. doi: 10.3390/insects12100889.
Acetyl-CoA, the precursor of sex pheromone biosynthesis in , is generated from glycolysis. As the first speed-limited enzyme in glycolysis, Hexokinase (HK) plays an important role in acetyl-CoA production. However, the function of HK in sex pheromone production remains unclear. This study employed as material to explore the role of HK in sex pheromone production. Results demonstrated that the transcription profile of in female moth pheromone glands (PGs) was consistent with the release fluctuation of sex pheromone. Interference of prevented the increase of acetyl-CoA content induced by PBAN. Therefore, knockdown of in female PGs caused significant decreases in ()-11-hexadecenal (11-16:Ald) production, female capability to attract males, and mating rate. Furthermore, sugar feeding (5% sugar) increased the transcription and enzymatic activity of HK. Pheromone biosynthesis activating neuropeptide (PBAN) signal phospho-activated HaHK in PGs and Sf9 cells via protein kinase A (PKA), as shown by pharmacological inhibitor analysis. In general, our study confirmed that PBAN/cAMP/PKA signal activated HaHK, in turn promoted glycolysis to ensure the supply of acetyl-CoA, and finally facilitated sex pheromone biosynthesis and subsequent mating behavior.
乙酰辅酶A是[昆虫名称]性信息素生物合成的前体，由糖酵解产生。己糖激酶（HK）作为糖酵解中的首个限速酶，在乙酰辅酶A的产生中起重要作用。然而，HK在性信息素产生中的功能仍不清楚。本研究以[昆虫名称]为材料，探究HK在性信息素产生中的作用。结果表明，[昆虫名称]雌性蛾类信息素腺（PGs）中的转录谱与性信息素的释放波动一致。对[昆虫名称]的干扰阻止了PBAN诱导的乙酰辅酶A含量增加。因此，雌性PGs中[昆虫名称]的敲低导致（Z）-11-十六碳烯醛（11-16:Ald）产量、雌性吸引雄性的能力和交配率显著下降。此外，喂食糖分（5%的糖）增加了HK的转录和酶活性。药理学抑制剂分析表明，信息素生物合成激活神经肽（PBAN）信号通过蛋白激酶A（PKA）在PGs和Sf9细胞中磷酸化激活HaHK。总体而言，我们的研究证实PBAN/cAMP/PKA信号激活HaHK，进而促进糖酵解以确保乙酰辅酶A的供应，最终促进性信息素生物合成及随后的交配行为。
