Silva Diego, Ceballos Ricardo, Arismendi Nolberto, Dalmon Anne, Vargas Marisol
Laboratorios de Virología y Patologías en Abejas, Facultad de Agronomía, Universidad de Concepción, Av. Vicente Méndez 595, Chillán 3780000, Chile.
Laboratorio de Ecología Química, Instituto de Investigaciones Agropecuarias, INIA Quilamapu, Av. Vicente Méndez 515, Chillán 3780000, Chile.
Insects. 2021 Oct 1;12(10):895. doi: 10.3390/insects12100895.
Insects have a highly sensitive sense of smell, allowing them to perform complex behaviors, such as foraging and peer recognition. Their sense of smell is based on the recognition of ligands and is mainly coordinated by odorant-binding proteins (OBPs). In , behavior can be affected by different pathogens, including deformed wing virus (DWV) and its variants. In particular, it has been shown that variant A of DWV (DWV-A) is capable of altering the ultra-cellular structure associated with olfactory activity. In this study was evaluated olfactory sensitivity and the expression of OBP genes in honey bees inoculated with DWV-A. Electroantennographic analyses (EAG) were carried out to determine the olfactory sensitivity to the essential oils and . The expression of nine antenna-specific OBP genes and DWV-A load in inoculated bees was also quantified by qPCR. We observed an inverse relationship between viral load and olfactory sensitivity and the expression of some OBP proteins. Thus, high viral loads reduced olfactory sensitivity to essential oils and the gene expression of the OBP2, OBP5, OBP11, and OBP12 proteins on the antennas of middle- and forager-age bees. These results suggest that DWV-A could have negative effects on the processes of aroma perception by worker bees, affecting their performance in tasks carried out in and outside the colony.
昆虫具有高度灵敏的嗅觉，这使它们能够执行复杂的行为，如觅食和同伴识别。它们的嗅觉基于配体识别，主要由气味结合蛋白（OBP）协调。在 中，行为会受到不同病原体的影响，包括变形翅病毒（DWV）及其变体。特别是，已表明DWV的A变体（DWV-A）能够改变与嗅觉活动相关的超细胞结构。在本研究中，评估了接种DWV-A的蜜蜂的嗅觉敏感性和OBP基因的表达。进行了触角电图分析（EAG）以确定对精油 和 的嗅觉敏感性。还通过qPCR对接种蜜蜂中九个触角特异性OBP基因的表达和DWV-A载量进行了定量。我们观察到病毒载量与嗅觉敏感性以及一些OBP蛋白的表达之间存在反比关系。因此，高病毒载量降低了对精油的嗅觉敏感性以及中龄和觅食龄蜜蜂触角上OBP2、OBP5、OBP11和OBP12蛋白的基因表达。这些结果表明，DWV-A可能对工蜂的香气感知过程产生负面影响，影响它们在蜂群内外执行任务的表现。
