间歇性低氧改变小鼠大脑和肝脏中时钟基因的昼夜节律表达。
Intermittent Hypoxia Alters the Circadian Expression of Clock Genes in Mouse Brain and Liver.
机构信息
Division of Pediatric Otolaryngology-Head and Neck Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Department of Pediatrics, Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
出版信息
Genes (Basel). 2021 Oct 16;12(10):1627. doi: 10.3390/genes12101627.
At least one-third of adults in the United States experience intermittent hypoxia (IH) due to health or living conditions. The majority of these adults suffer with sleep breathing conditions and associated circadian rhythm disorders. The impact of IH on the circadian clock is not well characterized. In the current study, we used an IH mouse model to understand the impact of IH on the circadian gene expression of the canonical clock genes in the central (the brain) and peripheral (the liver) tissues. Gene expression was measured using a Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). CircaCompare was used to evaluate the differential rhythmicity between normoxia and IH. Our observations suggested that the circadian clock in the liver was less sensitive to IH compared to the circadian clock in the brain.
在美国，至少有三分之一的成年人因健康或生活条件而经历间歇性低氧（IH）。这些成年人大多数患有睡眠呼吸障碍和相关的昼夜节律紊乱。IH 对生物钟的影响尚未得到很好的描述。在本研究中，我们使用 IH 小鼠模型来了解 IH 对中枢（大脑）和外周（肝脏）组织中经典时钟基因的昼夜基因表达的影响。使用定量逆转录聚合酶链反应（qRT-PCR）测量基因表达。CircaCompare 用于评估正常氧和 IH 之间的差异节律性。我们的观察结果表明，与大脑中的生物钟相比，肝脏中的生物钟对 IH 的敏感性较低。
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