Yang Kai-Ruei, Tsai Ming-Fong, Shieh Chwen-Jen, Arakawa Osamu, Dong Cheng-Di, Huang Chun-Yung, Kuo Chia-Hung
Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan.
Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
Foods. 2021 Oct 4;10(10):2363. doi: 10.3390/foods10102363.
Chondroitin sulfate (ChS) is usually used as an oral nutraceutical supplement, and has been popular in Asia, Europe, and United States for many years. In this study, a potential and sustainable source of ChS from jumbo squid () cartilage was explored; ultrasound-assisted extraction (UAE) was used to extract ChS from jumbo squid cartilage. The result of mass transfer coefficients based on Fick's law showed that UAE had higher mass transfer efficacy. The response surface methodology (RSM) combined with Box-Behnken design (BBD) was employed to evaluate the effects of the extraction parameters. The optimal conditions were extraction temperature of 52 °C, extraction time of 46 min, and NaOH concentration of 4.15%. The crude extract was precipitated by 50% ethanol, which obtained a purified ChS with 23.7% yield and 82.3% purity. The purified ChS measured by energy-dispersive X-ray spectroscopy (EDX) had a carbon to sulfur molar ratio of approximately 14:1. The FTIR, H, and C NMR confirmed jumbo squid ChS were present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate, with a 4S/6S ratio of 1.62. The results of this study provide an efficient process for production and purification of ChS, and are significant for the development and utilization of ChS from jumbo squid cartilage in the nutrient food or pharmaceutical industries.
硫酸软骨素(ChS)通常用作口服营养补充剂,多年来在亚洲、欧洲和美国都很受欢迎。在本研究中,探索了一种来自巨型鱿鱼软骨的潜在且可持续的ChS来源;采用超声辅助提取(UAE)从巨型鱿鱼软骨中提取ChS。基于菲克定律的传质系数结果表明,UAE具有更高的传质效率。采用响应面法(RSM)结合Box-Behnken设计(BBD)来评估提取参数的影响。最佳条件为提取温度52℃、提取时间46分钟和NaOH浓度4.15%。粗提物用50%乙醇沉淀,得到产率为23.7%、纯度为82.3%的纯化ChS。通过能量色散X射线光谱(EDX)测定的纯化ChS的碳硫摩尔比约为14:1。傅里叶变换红外光谱(FTIR)、氢核磁共振(H NMR)和碳核磁共振(C NMR)证实巨型鱿鱼ChS以硫酸软骨素-4-硫酸酯和硫酸软骨素-6-硫酸酯的形式存在,4S/6S比为1.62。本研究结果为ChS的生产和纯化提供了一种高效工艺,对于营养食品或制药行业中利用巨型鱿鱼软骨开发和利用ChS具有重要意义。