Department of Seafood Science, National Kaohsiung University of Science and Technology, 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung 81157, Taiwan, Republic of China.
Department of Seafood Science, National Kaohsiung University of Science and Technology, 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung 81157, Taiwan, Republic of China.
Int J Biol Macromol. 2020 Oct 1;160:677-687. doi: 10.1016/j.ijbiomac.2020.05.252. Epub 2020 May 30.
Squid pen sample was treated by high hydrostatic pressure (HHP) prior to the extraction of chitosan. The physicochemical and antioxidant activities of the chitosan obtained with HHP (HHP-CS) were compared with chitosan of untreated squid pen sample (UT-CS). The chitosan extraction yield was optimized using response surface methodology, and the optimum condition was achieved at pressure of 500 MPa, extraction time of 10 min, and 1% (w/w) acetate concentration. The maximum yield of chitosan sample from the chitin of squid pens treated by HHP reached 81.9%. Among the process variables, the combined effects of pressure and acetate concentration significantly enhanced the extraction of chitosan from squid pens. The HHP-CS was found to be significantly effective in enhancing the fat binding capacity, water binding capacity, and water solubility index. SEM image analysis suggested that the HHP-CS had a rough surface with high porosity, while UT-CS exhibited a smooth surface. In vitro antioxidant assay suggested that HHP-CS had significantly higher DPPH radical scavenging activity, greater reducing power, and a stronger ferrous ion chelating effect than did UT-CS. Therefore, HHP can be an excellent alternative method for improving the physicochemical properties and antioxidant activities of chitosan from squid pens.
鱿鱼笔样本在提取壳聚糖之前先经过高压处理（HPP）。将 HPP 处理（HHP-CS）后的壳聚糖与未经处理的鱿鱼笔样本壳聚糖（UT-CS）的物理化学性质和抗氧化活性进行了比较。采用响应面法优化了壳聚糖的提取产率，在压力为 500 MPa、提取时间为 10 min 和乙酸浓度为 1%（w/w）的最佳条件下，壳聚糖的提取率最高。经 HPP 处理的鱿鱼笔甲壳素的壳聚糖样品的最大产率达到 81.9%。在这些工艺变量中，压力和乙酸浓度的综合作用显著增强了鱿鱼笔壳聚糖的提取效果。结果表明，高压处理显著提高了壳聚糖的脂肪结合能力、水结合能力和水溶指数。SEM 图像分析表明，HHP-CS 具有粗糙的高孔隙表面，而 UT-CS 则具有光滑的表面。体外抗氧化试验表明，HHP-CS 对 DPPH 自由基的清除能力、还原能力和亚铁离子螯合能力均显著高于 UT-CS。因此，HPP 可以作为一种改善鱿鱼笔壳聚糖物理化学性质和抗氧化活性的极好替代方法。
