Wu Xu, Chen Feng, Zhao Xiaozhen, Pang Chengke, Shi Rui, Liu Changle, Sun Chengming, Zhang Wei, Wang Xiaodong, Zhang Jiefu
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Afairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Foods. 2021 Oct 11;10(10):2400. doi: 10.3390/foods10102400.
Oilseed-vegetable-dual-purpose (OVDP) rapeseed can effectively alleviate the land contradiction between crops and it supplements vegetable supplies in winter or spring. The soluble solids content (SSC) is an important index that is used to evaluate the quality and sugar content of fruits and vegetables. However, the genetic architecture underlying the SSC in shoots is still unclear. Here, quantitative trait loci (QTLs) for the SSC in shoots were investigated by performing linkage mapping using a recombinant inbred line population containing 189 lines. A germplasm set comprising 302 accessions was also used to conduct a genome-wide association study (GWAS). The QTL mapping revealed six QTLs located on chromosomes A01, A04, A08, and A09 in two experiments. Among them, two major QTLs, and , accounted for 12.92% and 10.18% of the phenotypic variance, respectively. In addition, eight single-nucleotide polymorphisms with phenotypic variances between 5.62% and 10.18% were identified by the GWAS method. However, no locus was simultaneously identified by QTL mapping and GWAS. We identified AH174 (7.55 °Brix and 7.9 °Brix), L166 (8.9 °Brix and 8.38 °Brix), and L380 (8.9 °Brix and 7.74 °Brix) accessions can be used as superior parents. These results provide valuable information that increases our understanding of the genetic control of SSC and will facilitate the breeding of high-SSC shoots.
油蔬两用型油菜能有效缓解作物间的土地矛盾，并补充冬春季节的蔬菜供应。可溶性固形物含量（SSC）是用于评估果蔬品质和含糖量的重要指标。然而，地上部SSC的遗传结构仍不清楚。在此，通过使用包含189个株系的重组自交系群体进行连锁图谱分析，研究了地上部SSC的数量性状位点（QTL）。还使用了一个由302份种质组成的种质集进行全基因组关联研究（GWAS）。QTL定位在两个实验中揭示了位于A01、A04、A08和A09染色体上的6个QTL。其中，两个主要QTL， 和 ，分别占表型变异的12.92%和10.18%。此外，通过GWAS方法鉴定出8个表型变异在5.62%至10.18%之间的单核苷酸多态性。然而，没有一个位点在QTL定位和GWAS中同时被鉴定出来。我们鉴定出AH174（7.55°白利糖度和7.9°白利糖度）、L166（8.9°白利糖度和8.38°白利糖度）和L380（8.9°白利糖度和7.74°白利糖度）株系可作为优良亲本。这些结果提供了有价值的信息，增进了我们对SSC遗传控制的理解，并将有助于高SSC地上部的育种。
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