一项全基因组关联研究揭示了菠菜中营养物质积累的遗传机制。

A Genome-Wide Association Study Reveals the Genetic Mechanisms of Nutrient Accumulation in Spinach.

机构信息

MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, China.

State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Genes (Basel). 2024 Jan 28;15(2):172. doi: 10.3390/genes15020172.

DOI:10.3390/genes15020172

PMID:38397162

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10887921/

Abstract

Spinach is a significant source of vitamins, minerals, and antioxidants. These nutrients make it delicious and beneficial for human health. However, the genetic mechanism underlying the accumulation of nutrients in spinach remains unclear. In this study, we analyzed the content of chlorophyll a, chlorophyll b, oxalate, nitrate, crude fiber, soluble sugars, manganese, copper, and iron in 62 different spinach accessions. Additionally, 3,356,182 high-quality, single-nucleotide polymorphisms were found using resequencing and used in a genome-wide association study. A total of 2077 loci were discovered that significantly correlated with the concentrations of the nutritional elements. Data mining identified key genes in these intervals for four traits: chlorophyll, oxalate, soluble sugar, and Fe. Our study provides insights into the genetic architecture of nutrient variation and facilitates spinach breeding for good nutrition.

摘要

菠菜是维生素、矿物质和抗氧化剂的重要来源。这些营养物质使它既美味又有益于人类健康。然而，菠菜中营养物质积累的遗传机制尚不清楚。在这项研究中，我们分析了 62 个不同菠菜品种中叶绿素 a、叶绿素 b、草酸盐、硝酸盐、粗纤维、可溶性糖、锰、铜和铁的含量。此外，我们通过重测序发现了 3356182 个高质量的单核苷酸多态性，并用于全基因组关联研究。总共发现了 2077 个与营养元素浓度显著相关的位点。数据挖掘确定了这四个性状（叶绿素、草酸盐、可溶性糖和铁）在这些区间的关键基因。我们的研究为营养物质变化的遗传结构提供了深入的了解，并为培育富含营养的菠菜品种提供了便利。