Injury Prevention Research Center, Shantou University Medical College, Shantou 515041, China.
School of Public Health, Shantou University, Shantou 515041, China.
Int J Environ Res Public Health. 2021 Oct 15;18(20):10828. doi: 10.3390/ijerph182010828.
The catechol-O-methyltransferase (COMT) is a candidate gene to provide promising evidence of psychiatric disorders, but there is a knowledge gap between the genetic factor and multiple physical activity-related injuries (PARIs). The aim of this study was to explore the contribution of COMT to the risk of PARIs among university students in the Chinese Han population. We can further search for the intrinsic risk factors for the occurrence of multiple physical activity injuries and provide a scientific basis for early screening and precise intervention for the high-risk group of college students with multiple PARIs. A 1:1 matched case-control study of 61 PARIs cases and 61 healthy controls were carried out. DNA samples of the participants were isolated from saliva and genotyped on eight SNPs of the COMT gene (rs9265, rs4680, rs6269, rs4818, rs4633, rs165655, rs165656, and rs165722) using the MALDI-TOF MS method. We found that rs6269 and rs4818 were significantly associated with PARIs, and rs6269-GG and rs4818-GG contributed to the reduced risk of PARIs. Further haplotype analysis showed a four-marker C-G-C-G haplotype (rs165722-rs6269-rs4633-rs4818) acted with a protective role in the development of PARIs ( = 0.037; OR: 0.474, 95% CI: 0.269 to 0.834). However, the interactions between club membership and rs6269 or rs4818 would significantly increase the risk of PARIs (both < 0.001, OR: 5.121 and 4.977, respectively). This is the first study to find the contribution of COMT to PARIs occurrence, suggesting that the COMT polymorphisms and the gene-environment interactions may alter the risk of PARIs.
儿茶酚-O-甲基转移酶（COMT）是提供精神疾病有希望的证据的候选基因，但遗传因素与多种身体活动相关损伤（PARIs）之间存在知识差距。本研究旨在探讨 COMT 在中国汉族人群大学生 PARIs 风险中的作用。我们可以进一步寻找多种身体活动损伤发生的内在风险因素，为具有多种 PARIs 的大学生高危人群的早期筛查和精确干预提供科学依据。对 61 例 PARIs 病例和 61 例健康对照进行了 1:1 匹配的病例对照研究。从唾液中分离参与者的 DNA 样本，并使用 MALDI-TOF MS 方法对 COMT 基因的 8 个 SNPs（rs9265、rs4680、rs6269、rs4818、rs4633、rs165655、rs165656 和 rs165722）进行基因分型。我们发现 rs6269 和 rs4818 与 PARIs 显著相关，rs6269-GG 和 rs4818-GG 降低 PARIs 的风险。进一步的单倍型分析显示，四个标记物 C-G-C-G 单倍型（rs165722-rs6269-rs4633-rs4818）在 PARIs 的发展中起保护作用（ = 0.037；OR：0.474，95%CI：0.269 至 0.834）。然而，俱乐部会员资格与 rs6269 或 rs4818 之间的相互作用会显著增加 PARIs 的风险（均 < 0.001，OR：5.121 和 4.977）。这是第一项发现 COMT 对 PARIs 发生贡献的研究，表明 COMT 多态性和基因-环境相互作用可能改变 PARIs 的风险。
