光化性角化病和皮肤鳞状细胞癌患者血清唾液酸化的变化

Serum Sialylation Changes in Actinic Keratosis and Cutaneous Squamous Cell Carcinoma Patients.

作者信息

Tampa Mircea, Nicolae Ilinca, Mitran Cristina Iulia, Mitran Madalina Irina, Ene Cosmin, Matei Clara, Georgescu Simona Roxana, Ene Corina Daniela

机构信息

Department of Dermatology, 'Carol Davila' University of Medicine and Pharmacy, 020021 Bucharest, Romania.

Department of Dermatology, 'Victor Babes' Clinical Hospital for Infectious Diseases, 030303 Bucharest, Romania.

出版信息

J Pers Med. 2021 Oct 15;11(10):1027. doi: 10.3390/jpm11101027.

DOI:10.3390/jpm11101027

PMID:34683168

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8538811/

Abstract

Cutaneous squamous cell carcinoma (cSCC), a malignant proliferation of the cutaneous epithelium, is the second most common skin cancer after basal cell carcinoma (BCC). Unlike BCC, cSCC exhibits a greater aggressiveness and the ability to metastasize to any organ in the body. Chronic inflammation and immunosuppression are important processes linked to the development of cSCC. The tumor can occur de novo or from the histological transformation of preexisting actinic keratoses (AK). Malignant cells exhibit a higher amount of sialic acid in their membranes than normal cells, and changes in the amount, type, or linkage of sialic acid in malignant cell glycoconjugates are related to tumor progression and metastasis. The aim of our study was to investigate the sialyation in patients with cSCC and patients with AK. We have determined the serum levels of total sialic acid (TSA), lipid-bound sialic acid (LSA), beta-galactoside 2,6-sialyltransferase I (ST6GalI), and neuraminidase 3 (NEU3) in 40 patients with cSCC, 28 patients with AK, and 40 healthy subjects. Data analysis indicated a significant increase in serum levels of TSA ( < 0.001), LSA ( < 0.001), ST6GalI ( < 0.001), and NEU3 ( < 0.001) in the cSCC group compared to the control group, whereas in patients with AK only the serum level of TSA was significantly higher compared to the control group ( < 0.001). When the cSCC and AK groups were compared, significant differences between the serum levels of TSA ( < 0.001), LSA ( < 0.001), ST6GalI ( < 0.001) and NEU3 ( < 0.001) were found. The rate of synthesis of sialoglycoconjugates and their rate of enzymatic degradation, expressed by the ST6GalI/NEU3 ratio, is 1.64 times lower in the cSCC group compared to the control group ( < 0.01) and 1.53 times lower compared to the AK group ( < 0.01). The tumor diameter, depth of invasion, and Ki67 were associated with higher levels of TSA and LSA. These results indicate an aberrant sialylation in cSCC that correlates with tumor aggressiveness.

摘要

皮肤鳞状细胞癌（cSCC）是皮肤上皮的恶性增殖，是仅次于基底细胞癌（BCC）的第二常见皮肤癌。与BCC不同，cSCC具有更强的侵袭性，能够转移至身体的任何器官。慢性炎症和免疫抑制是与cSCC发生相关的重要过程。肿瘤可原发产生，也可由先前存在的光化性角化病（AK）组织学转化而来。恶性细胞细胞膜中的唾液酸含量高于正常细胞，恶性细胞糖缀合物中唾液酸的含量、类型或连接方式的变化与肿瘤进展和转移相关。我们研究的目的是调查cSCC患者和AK患者的唾液酸化情况。我们测定了40例cSCC患者、28例AK患者和40名健康受试者血清中总唾液酸（TSA）、脂质结合唾液酸（LSA）、β-半乳糖苷2,6-唾液酸转移酶I（ST6GalI）和神经氨酸酶3（NEU3）的水平。数据分析表明，与对照组相比，cSCC组血清TSA（<0.001）、LSA（<0.001）、ST6GalI（<0.001）和NEU3（<0.001）水平显著升高，而在AK患者中，仅血清TSA水平与对照组相比显著升高（<0.001）。当比较cSCC组和AK组时，发现血清TSA（<0.001）、LSA（<0.001）、ST6GalI（<0.001）和NEU3（<0.001）水平存在显著差异。以ST6GalI/NEU3比值表示的唾液酸糖缀合物合成速率及其酶促降解速率，cSCC组比对照组低1.64倍（<0.01），比AK组低1.53倍（<0.01）。肿瘤直径、浸润深度和Ki67与较高水平的TSA和LSA相关。这些结果表明cSCC中存在异常的唾液酸化，且与肿瘤侵袭性相关。