Soliman Mariano, Bocchini Marika, Stein Juliana, Ortiz Juan Pablo A, Albertini Emidio, Delgado Luciana
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Rosario S2125ZAA, Zavalla, Argentina.
Department Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy.
Plants (Basel). 2021 Oct 4;10(10):2100. doi: 10.3390/plants10102100.
In angiosperms, gametophytic apomixis (clonal reproduction through seeds) is strongly associated with polyploidy and hybridization. The trait is facultative and its expressivity is highly variable between genotypes. Here, we used an F progeny derived from diploid apomictic (aposporic) genotypes of and two F families, derived from F hybrids with different apospory expressivity (%AES), to analyze the influence of the environment and the transgenerational transmission of the trait. In addition, AFLP markers were developed in the F population to identify genomic regions associated with the %AES. Cytoembryological analyses showed that the %AES was significantly influenced by different environments, but remained stable across the years. F and F progenies showed a wide range of %AES variation, but most hybrids were not significantly different from the parental genotypes. Maternal and paternal genetic linkage maps were built covering the ten expected linkage groups (LG). A single-marker analysis detected at least one region of 5.7 cM on LG3 that was significantly associated with apospory expressivity. Our results underline the importance of environmental influence in modulating apospory expressivity and identified a genomic region associated with apospory expressivity at the diploid level.
在被子植物中，配子体无融合生殖（通过种子进行克隆繁殖）与多倍体和杂交密切相关。该性状是兼性的，其表达在不同基因型之间高度可变。在这里，我们使用了来自二倍体无融合生殖（无孢子生殖）基因型的F子代，以及来自具有不同无孢子生殖表达率（%AES）的F杂种的两个F家系，来分析环境的影响和该性状的跨代传递。此外，在F群体中开发了AFLP标记，以鉴定与%AES相关的基因组区域。细胞胚胎学分析表明，%AES受不同环境的显著影响，但多年来保持稳定。F和F子代表现出广泛的%AES变异，但大多数杂种与亲本基因型没有显著差异。构建了覆盖十个预期连锁群（LG）的母本和父本遗传连锁图谱。单标记分析在LG3上检测到至少一个5.7 cM的区域，该区域与无孢子生殖表达率显著相关。我们的结果强调了环境影响在调节无孢子生殖表达率中的重要性，并在二倍体水平上鉴定了一个与无孢子生殖表达率相关的基因组区域。
