National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, People's Republic of China.
BMC Vet Res. 2021 Oct 22;17(1):335. doi: 10.1186/s12917-021-03045-y.
N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity.
In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro.
Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.
N-乙酰基转移酶 13（NAT13）是 ARD1A-NARG1 复合物的一个可能的催化组成部分，具有 α（N 端）乙酰基转移酶活性。
在这项研究中，从血吸虫 cDNA 中分离出编码日本血吸虫 NAT13（SjNAT13）的全长 cDNA。SjNAT13 的 621bp 开放阅读框编码 206 个氨基酸的多肽。实时 PCR 分析显示 SjNAT13 在所有测试的发育阶段都有表达。在尾蚴和 21 日龄蠕虫中，转录水平最高，雄性成虫中的转录水平高于雌性成虫。rSjNAT13 蛋白诱导高水平的抗 rSjNAT13 IgG 抗体。在两项独立的免疫保护试验中，rSjNAT13 分别使肝内卵数减少了 24.23%和 24.47%。RNA 干扰（RNAi）结果表明，小干扰 RNA（siRNA）Sj-514 显著降低了蠕虫中的 SjNAT13 转录水平，并减少了体外产卵。
因此，rSjNAT13 可能在血吸虫的发育和繁殖中发挥重要作用。
