Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Teaching Hospital, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
J Comp Pathol. 2021 Oct;188:44-51. doi: 10.1016/j.jcpa.2021.08.004. Epub 2021 Sep 24.
Following isolation of pseudorabies virus (PRV) from two hunting dogs in Oita prefecture, Japan, we investigated the PRV antigen distribution in the tissues of the infected animals. At necropsy, PRV-associated lesions included facial oedema, tonsillar and meningeal congestion, blotchy haemorrhages on the pericardium and mitral valves, and incomplete splenic contraction in one dog, with less prominent findings in the other dog. Multiple pulmonary nodules were seen in both cases, caused by the diploid form of Paragonimus westermani lung flukes, as confirmed by PCR-restriction fragment length polymorphism and gene sequencing analyses. Histological examination revealed that the PRV infection was associated with lesions of non-suppurative encephalitis in the brainstem. PRV antigen was detected in the cerebrum, cerebellum, brainstem, submucosal and myenteric plexuses, and mononuclear cells, mainly in the bone marrow, lymph nodes, tonsils and spleen tissues. There was evidence of PRV dissemination to the brain via the trigeminal or olfactory routes, in addition to possible spread to lymphoid organs via infected mononuclear cells.
在日本大分县从两只猎犬中分离出伪狂犬病病毒 (PRV) 后，我们调查了感染动物组织中的 PRV 抗原分布。尸检时，与 PRV 相关的病变包括面部水肿、扁桃体和脑膜充血、心包和二尖瓣上斑驳状出血，以及一只狗的脾脏不完全收缩，另一只狗的病变不那么明显。两只狗都出现了多发性肺结节，这是由二倍体型卫氏并殖吸虫肺吸虫引起的，通过 PCR-限制性片段长度多态性和基因测序分析得到证实。组织学检查显示，PRV 感染与脑干非化脓性脑炎病变有关。PRV 抗原在大脑、小脑、脑干、黏膜下和肌间神经丛以及单核细胞中被检测到，主要在骨髓、淋巴结、扁桃体和脾脏组织中。有证据表明 PRV 通过三叉神经或嗅觉途径传播到大脑，除了通过感染的单核细胞向淋巴器官传播之外。
